Linked Life Data

10393251

Source:http://linkedlifedata.com/resource/pubmed/id/10393251

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-8-19
pubmed:databankReference
pubmed:abstractText
HOX11 is a gene encoding a homeobox protein which is found to be deregulated in T-cell acute lymphoblastic leukaemia (T-ALL). As a basis for studying the mechanism of deregulation of HOX11 expression in leukaemia, the locus containing the HOX11 proto-oncogene at 10q24 was cloned from a genomic P1 Artificial Chromosome (PAC) library. The PAC clone with an insert size of 120kb was isolated and mapped by restriction analysis. A series of contiguous subclones were then obtained which span 20kb surrounding the HOX11 gene. These subclones were used to sequence across the entire 20kb region to the 3' boundary of the PAC insert. This work provides for the first time the full intron and 5' non-coding sequences of the HOX11 gene which will aid the identification of novel transcriptional control elements which may be involved in silencing HOX11 expression in normal cells. The sequence information was also used to search for novel large open reading frames (ORFs). One such ORF (1.1kb) would encode a protein of at least 39kDa. This basic protein (pI, 12.5) would be very proline rich and could potentially encode a novel transcription factor. In order to establish if this ORF corresponds to a bona fide transcribed gene, RT-PCR analysis was performed. The mRNA for this protein is expressed in the T-ALL cell line Jurkat and has been designated HUG1, for HOX11 Upstream Gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
234
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
169-76
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Sequence of 10q24 locus surrounding the HOX11 oncogene reveals a new gene HUG1 expressed in a T-ALL cell line.
pubmed:affiliation
TVW Telethon Institute for Child Health Research,1 PO Box 855, West Perth, Western Australia 6872, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't