Source:http://linkedlifedata.com/resource/pubmed/id/10390535
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
14
|
| pubmed:dateCreated |
1999-9-3
|
| pubmed:abstractText |
We have identified a MAR/SAR recognition signature (MRS) which is common to a large group of matrix and scaffold attachment regions. The MRS is composed of two degenerate sequences (AATAAYAA and AWWRTAANNWWGNNNC) within close proximity. Analysis of >300 kb of genomic sequence from a variety of eukaryotic organisms shows that the MRS faithfully predicts 80% of MARs and SARs. In each case where we find a MRS, the corresponding DNA region binds specifically to the nuclear scaffold. Although all MRSs are associated with a SAR, not all known SARs and MARs contain a MRS, suggesting that at least two classes exist, one containing a MRS, the other not. Evidence is presented that the two sequence elements of the bipartite MRS occupy a position on the nucleosome near the dyad axis, together creating a putative protein binding site. The identification of a MAR- and SAR-associated DNA element is an important step forward towards understanding the molecular mechanisms of these elements. It will allow: (i) analysis of the genomic location of SARs, e.g. in relationship to genes, based on sequence information alone, rather than on the basis of an elaborate biochemical assay; (ii) identification and analysis of proteins that specifically bind to the MRS.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Globins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleosomes
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0305-1048
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
27
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2924-30
|
| pubmed:dateRevised |
2008-11-20
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| pubmed:meshHeading |
pubmed-meshheading:10390535-Animals,
pubmed-meshheading:10390535-Antigens, Nuclear,
pubmed-meshheading:10390535-Arabidopsis,
pubmed-meshheading:10390535-Base Sequence,
pubmed-meshheading:10390535-Binding Sites,
pubmed-meshheading:10390535-Caenorhabditis elegans,
pubmed-meshheading:10390535-Cell Nucleus,
pubmed-meshheading:10390535-Conserved Sequence,
pubmed-meshheading:10390535-DNA,
pubmed-meshheading:10390535-DNA-Binding Proteins,
pubmed-meshheading:10390535-Eukaryotic Cells,
pubmed-meshheading:10390535-Genome,
pubmed-meshheading:10390535-Globins,
pubmed-meshheading:10390535-Humans,
pubmed-meshheading:10390535-Locus Control Region,
pubmed-meshheading:10390535-Nuclear Matrix,
pubmed-meshheading:10390535-Nuclear Proteins,
pubmed-meshheading:10390535-Nucleosomes,
pubmed-meshheading:10390535-Regulatory Sequences, Nucleic Acid
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
A bipartite sequence element associated with matrix/scaffold attachment regions.
|
| pubmed:affiliation |
E. C. Slater Instituut, BioCentrum Amsterdam, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|