Linked Life Data

10384147

Source:http://linkedlifedata.com/resource/pubmed/id/10384147

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-7-15
pubmed:abstractText
It is well established that melanoma cells express Ags that are recognized by autologous T cells in vitro. Tumor-infiltrating lymphocytes in situ comprise clonotypic T cells, suggesting that their expansion is driven by Ag stimulation. Still, little is known about the detailed characteristics of the in situ T cell response. In the present study, we scrutinized this response by analyzing multiple metastatic lesions for the presence of clonotypic T cells. This approach was chosen to distinguish whether the clonal T cell expansion occurs as a systemic or localized phenomenon. TCR clonotype mapping of six s.c. metastases from two patients revealed the presence of multiple (from 40 to >60) clonotypic T cells in all lesions. Clonotypic T cells were present in TCR beta-variable regions expressed both at high and low levels. Comparison of the T cell clonotypes present in different lesions from individual patients demonstrated that, in general, clonotypes were exclusively detected in a single lesion. Hence, anti-melanoma T cell responses are much more heterogeneous than previously anticipated and accommodate a predominance of strictly localized T cell clonotypes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
443-7
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:10384147-Antigens, Neoplasm, pubmed-meshheading:10384147-Cell Differentiation, pubmed-meshheading:10384147-Clone Cells, pubmed-meshheading:10384147-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:10384147-Humans, pubmed-meshheading:10384147-Lymphocyte Count, pubmed-meshheading:10384147-Lymphocytes, Tumor-Infiltrating, pubmed-meshheading:10384147-Melanoma, pubmed-meshheading:10384147-Melanoma-Specific Antigens, pubmed-meshheading:10384147-Neoplasm Proteins, pubmed-meshheading:10384147-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:10384147-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10384147-Sequence Analysis, DNA, pubmed-meshheading:10384147-Skin Neoplasms, pubmed-meshheading:10384147-T-Lymphocyte Subsets, pubmed-meshheading:10384147-Transcription, Genetic, pubmed-meshheading:10384147-Tumor Markers, Biological
pubmed:year
1999
pubmed:articleTitle
In situ T cell responses against melanoma comprise high numbers of locally expanded T cell clonotypes.
pubmed:affiliation
Department of Tumor Cell Biology, Institute of Cancer Biology, Danish Cancer Society, Copenhagen. ps@cancer.dk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't