Linked Life Data

10384129

Source:http://linkedlifedata.com/resource/pubmed/id/10384129

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-7-15
pubmed:abstractText
TCR alpha (TCRA) expression was examined in RNA samples from PBMC and isolated populations of CD4+, CD8+, and DN T cells from 15 healthy individuals. The expressed TCR repertoire was surveyed using spectratype analysis, a technique that displays the distribution of complementarity determining region 3 (CDR3) lengths for each TCRAV gene family. The results revealed the presence of unusual populations of double-negative (DN; CD4-CD8-CD3+) T cells that express invariant or conserved TCRAV4A, AV7, AV19, and AV24 chains. Each of the conserved TCRA families was over-represented in >70% of the individuals studied, and all individuals expressed at least one of the over-represented TCRAV families. Over-represented conserved AV4A or AV7 sequences were also present in CD8+ T cells from most donors. The extent of TCRA sequence conservation is unparalleled. TCRAV4A, AV19, and AV24 sequences were invariant, although AV4A and AV19 transcripts contained N region additions. TCRAV24 transcripts derived from the direct juxtaposition of V and J gene segments. TCRAV7 sequences showed some diversity in two amino acids encoded at junctions of V and J gene segments. Although derivation of DN T cells with conserved TCRA chains is puzzling, the wide-spread expression of these unusual cells suggests an important function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
301-11
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10384129-Adult, pubmed-meshheading:10384129-Amino Acid Sequence, pubmed-meshheading:10384129-Antigens, CD3, pubmed-meshheading:10384129-Antigens, CD4, pubmed-meshheading:10384129-Antigens, CD56, pubmed-meshheading:10384129-Antigens, CD8, pubmed-meshheading:10384129-CD8-Positive T-Lymphocytes, pubmed-meshheading:10384129-Conserved Sequence, pubmed-meshheading:10384129-Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor, pubmed-meshheading:10384129-Humans, pubmed-meshheading:10384129-Immunophenotyping, pubmed-meshheading:10384129-Killer Cells, Natural, pubmed-meshheading:10384129-Molecular Sequence Data, pubmed-meshheading:10384129-Polymerase Chain Reaction, pubmed-meshheading:10384129-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:10384129-T-Lymphocyte Subsets, pubmed-meshheading:10384129-Transcription, Genetic
pubmed:year
1999
pubmed:articleTitle
Invariant or highly conserved TCR alpha are expressed on double-negative (CD3+CD4-CD8-) and CD8+ T cells.
pubmed:affiliation
Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, Rockville, MD 20852, USA.
pubmed:publicationType
Journal Article, Comparative Study