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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-7-15
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pubmed:abstractText |
CD23, also known as the low affinity IgE receptor (FcepsilonRII), has been hypothesized to have a role in IgE regulation. A new CD23 transgenic mouse was generated using the MHC class I promoter and IgH enhancer to further test the hypothesis that CD23 plays a role in the down-regulation of IgE. Study of three founder lines by FACS showed overexpression to varying extents on both B and T lymphocytes. No alterations in lymphocyte populations was observed. All three founder lines exhibited strong suppression of IgE in response to DNP-keyhole limpet hemocyanin/alum and Nippostrongylus brasiliensis infection compared with that in parental or littermate controls. The founder line exhibiting the highest level of suppression also was less susceptible to Ag-induced systemic anaphylactic shock. Overall, the data support the concept that enhancing CD23 levels can be used to suppress IgE-mediated disease. The mechanism involves decreased IgE synthesis, because the serum half-life of IgE was not altered in transgenics, and enzyme-linked immunospot analysis demonstrated lower IgE-producing cells stimulated by injection of anti-IgD. Transgenics also exhibited significantly decreased IgG1 responses and exhibited lower levels of all Ig isotypes, although this was more variable in different founder lines.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,4-dinitrophenyl keyhole limpet...,
http://linkedlifedata.com/resource/pubmed/chemical/Alum Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Helminth,
http://linkedlifedata.com/resource/pubmed/chemical/Haptens,
http://linkedlifedata.com/resource/pubmed/chemical/Hemocyanin,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgE,
http://linkedlifedata.com/resource/pubmed/chemical/aluminum sulfate,
http://linkedlifedata.com/resource/pubmed/chemical/anti-IgD
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
163
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
217-23
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10384119-Alum Compounds,
pubmed-meshheading:10384119-Anaphylaxis,
pubmed-meshheading:10384119-Animals,
pubmed-meshheading:10384119-Antibodies, Anti-Idiotypic,
pubmed-meshheading:10384119-Antibodies, Helminth,
pubmed-meshheading:10384119-Half-Life,
pubmed-meshheading:10384119-Haptens,
pubmed-meshheading:10384119-Hemocyanin,
pubmed-meshheading:10384119-Immune Tolerance,
pubmed-meshheading:10384119-Immunoglobulin E,
pubmed-meshheading:10384119-Immunoglobulins,
pubmed-meshheading:10384119-Immunophenotyping,
pubmed-meshheading:10384119-Injections, Intravenous,
pubmed-meshheading:10384119-Injections, Subcutaneous,
pubmed-meshheading:10384119-Male,
pubmed-meshheading:10384119-Mice,
pubmed-meshheading:10384119-Mice, Inbred BALB C,
pubmed-meshheading:10384119-Mice, Inbred C57BL,
pubmed-meshheading:10384119-Mice, Transgenic,
pubmed-meshheading:10384119-Nippostrongylus,
pubmed-meshheading:10384119-Receptors, IgE,
pubmed-meshheading:10384119-Strongylida Infections
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pubmed:year |
1999
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pubmed:articleTitle |
Humoral response suppression observed with CD23 transgenics.
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pubmed:affiliation |
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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