Linked Life Data

10382232

Source:http://linkedlifedata.com/resource/pubmed/id/10382232

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-7-29
pubmed:abstractText
Proliferation of alveolar type II cells is critical for restoration of the integrity of alveolar epithelium in alveolar injuries caused by a number of different aetiologies. Because effects of inflammatory cytokines on the proliferation of alveolar type II cells are not clear, we investigated the effects of interleukin-1 beta (IL-1 beta) on [3H]-thymidine incorporation into DNA in rat alveolar type II cells in primary culture. Interleukin-1 beta enhanced the [3H]-thymidine incorporation dose and time dependently. The increase of [3H]-thymidine incorporation was observed in parallel with increased number of rat alveolar type II cells. The effect of IL-1 beta on [3H]-thymidine incorporation was additive to effects of growth factors which were known to act as mitogenic factors for type II cells. Anti-interleukin-1 beta antibody or IL-1 receptor antagonist partially inhibited the effects of IL-1 beta on [3H]-thymidine incorporation. Their combination completely inhibited the effects of IL-1 beta. In the absence of IL-1 beta, the combination inhibited the [3H]-thymidine incorporation to a level under that in the control. Isolated alveolar type II cells were immunocytochemically stained positive with anti-IL-1 beta antibody. Reverse transcriptase-polymerase chain reaction (RT-PCR) showed the presence of the mRNA for IL-1 beta in cultured alveolar type II cells. These results demonstrate that exogenous IL-1 beta stimulates DNA synthesis in alveolar type II cells and that the cells also produce IL-1 beta endogenously and suggest that endogenous IL-1 beta may mediate basal DNA synthesis of alveolar type II cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1323-7799
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
139-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Effects of interleukin-1 beta on DNA synthesis in rat alveolar type II cells in primary culture.
pubmed:affiliation
Department of Internal Medicine, Kanazawa Medical University, Ishikawa, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't