Linked Life Data

10381890

Source:http://linkedlifedata.com/resource/pubmed/id/10381890

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1999-7-16
pubmed:abstractText
To determine whether opioid receptors (ORs) are involved in the delayed cardioprotection of ischemic preconditioning (IP), the effect of severe metabolic inhibition (MI) with a glucose-free buffer that contained sodium cyanide and 2-deoxy-D-glucose on the viability of isolated rat ventricular myocytes was first determined 20 hours after preconditioning with a sublethal metabolic inhibition (MIP) with a glucose-free buffer that contained 2-deoxy-D-glucose and lactate for 30 minutes in the presence of OR antagonists. With the use of trypan blue exclusion as an index of cell viability, severe MI killed >60% of the cells and the value increased significantly after MIP. In the presence of 5x10(-6) mol/L nor-binaltorphimine (nor-BNI), a selective kappa-OR antagonist, but not 5x10(-6) mol/L CTOP, a selective mu-OR antagonist, or 5x10(-6) mol/L naltrindole, a selective delta-OR antagonist, the cardioprotection of MIP was significantly attenuated. To verify the role of kappa-OR, we studied the effects of severe MI after pretreatment with the kappa-OR agonist U50,488H (UP) for 30 minutes. U50,488H at 3x10(-6) to 1x10(-4) mol/L increased cell viability concentration-dependently with an EC50 of 3.311x10(-6) mol/L. In the presence of 5x10(-6) nor-BNI, the cardioprotection of UP (3x10(-5) mol/L) was blocked. A time course study showed that UP-induced cardioprotection occurred in 2 windows: the first occurred approximately 1 hour later and the other occurred 16 to 20 hours later. Additional studies on cell contraction and intracellular Ca2+ ([Ca2+]i) revealed that both UP and MIP attenuated the inhibitory effects of severe MI on contractility and electrically induced [Ca2+]i transient in single ventricular myocytes. On blockade of protein kinase C, the delayed cardioprotections of UP and MIP were significantly attenuated. In conclusion, the results of the present study have provided evidence that kappa-OR mediates the cardioprotection of MIP, which may involve protein kinase C and [Ca2+]i.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0009-7330
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1388-95
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10381890-3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-ben..., pubmed-meshheading:10381890-Analgesics, Non-Narcotic, pubmed-meshheading:10381890-Animals, pubmed-meshheading:10381890-Calcium, pubmed-meshheading:10381890-Cell Survival, pubmed-meshheading:10381890-Electrophysiology, pubmed-meshheading:10381890-Energy Metabolism, pubmed-meshheading:10381890-Heart Ventricles, pubmed-meshheading:10381890-Ischemic Preconditioning, Myocardial, pubmed-meshheading:10381890-Membrane Potentials, pubmed-meshheading:10381890-Muscle Fibers, Skeletal, pubmed-meshheading:10381890-Myocardial Contraction, pubmed-meshheading:10381890-Myocardial Ischemia, pubmed-meshheading:10381890-Myocardium, pubmed-meshheading:10381890-Naltrexone, pubmed-meshheading:10381890-Narcotic Antagonists, pubmed-meshheading:10381890-Protein Kinase C, pubmed-meshheading:10381890-Rats, pubmed-meshheading:10381890-Rats, Sprague-Dawley, pubmed-meshheading:10381890-Receptors, Opioid, kappa, pubmed-meshheading:10381890-Trypan Blue
pubmed:year
1999
pubmed:articleTitle
Cardioprotection of preconditioning by metabolic inhibition in the rat ventricular myocyte. Involvement of kappa-opioid receptor.
pubmed:affiliation
Department of Physiology and Institute of Cardiovascular Science and Medicine, Faculty of Medicine, The University of Hong Kong, China.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't