Source:http://linkedlifedata.com/resource/pubmed/id/10381536
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
1999-7-23
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| pubmed:abstractText |
Genetic modification of hematopoietic stem cells with genes that inhibit replication of human immunodeficiency virus-1 (HIV-1) could lead to development of T lymphocytes and monocytic cells resistant to HIV-1 infection after transplantation. We performed a clinical trial to evaluate the safety and feasibility of this procedure, using bone marrow from four HIV-1-infected pediatric subjects (ages 8 to 17 years). We obtained bone marrow, isolated CD34(+) cells, performed in vitro transduction with a retroviral vector carrying a rev-responsive element (RRE) decoy gene, and reinfused the cells into these subjects with no evidence of adverse effects. The levels of gene-containing leukocytes in peripheral blood samples in the 1 year after gene transfer/cell infusion have been extremely low. These observations support the potential of performing gene therapy for HIV-1 using hematopoietic cells, but emphasize the need for improved gene transfer techniques.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0006-4971
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| pubmed:author |
pubmed-author:Aguilar-CordovaEE,
pubmed-author:BahnerII,
pubmed-author:BauerGG,
pubmed-author:BrodtPP,
pubmed-author:CarbonaroD ADA,
pubmed-author:ChurchJJ,
pubmed-author:DolanCC,
pubmed-author:FozMM,
pubmed-author:HadenEE,
pubmed-author:KearnsKK,
pubmed-author:KohnD BDB,
pubmed-author:MayD JDJ,
pubmed-author:PuriPP,
pubmed-author:RothschildJ CJC,
pubmed-author:SalataCC,
pubmed-author:ValdesYY,
pubmed-author:WetterCC,
pubmed-author:WilsonKK,
pubmed-author:ZhouCC
|
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
94
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
368-71
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:10381536-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:10381536-Adolescent,
pubmed-meshheading:10381536-Cell Differentiation,
pubmed-meshheading:10381536-Child,
pubmed-meshheading:10381536-Gene Therapy,
pubmed-meshheading:10381536-Gene Transfer Techniques,
pubmed-meshheading:10381536-Genes, rev,
pubmed-meshheading:10381536-Genetic Vectors,
pubmed-meshheading:10381536-HIV Long Terminal Repeat,
pubmed-meshheading:10381536-HIV-1,
pubmed-meshheading:10381536-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:10381536-Hematopoietic Stem Cells,
pubmed-meshheading:10381536-Humans,
pubmed-meshheading:10381536-Pilot Projects,
pubmed-meshheading:10381536-Retroviridae,
pubmed-meshheading:10381536-T-Lymphocytes,
pubmed-meshheading:10381536-Virus Replication
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
A clinical trial of retroviral-mediated transfer of a rev-responsive element decoy gene into CD34(+) cells from the bone marrow of human immunodeficiency virus-1-infected children.
|
| pubmed:affiliation |
Division of Research Immunology/Bone Marrow Transplantation, Childrens Hospital Los Angeles, CA 90027, USA. dkohn@chla.usc.edu
|
| pubmed:publicationType |
Journal Article,
Clinical Trial,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|