| pubmed-article:10376844 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10376844 | lifeskim:mentions | umls-concept:C0004611 | lld:lifeskim |
| pubmed-article:10376844 | lifeskim:mentions | umls-concept:C0995501 | lld:lifeskim |
| pubmed-article:10376844 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:10376844 | lifeskim:mentions | umls-concept:C1513380 | lld:lifeskim |
| pubmed-article:10376844 | lifeskim:mentions | umls-concept:C0037697 | lld:lifeskim |
| pubmed-article:10376844 | lifeskim:mentions | umls-concept:C0439542 | lld:lifeskim |
| pubmed-article:10376844 | pubmed:dateCreated | 1999-9-21 | lld:pubmed |
| pubmed-article:10376844 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376844 | pubmed:abstractText | The recA gene of the purple non-sulfur bacterium Rhodopseudomonas palustris no. 7 was isolated by a PCR-based method and sequenced. The complete nucleotide sequence consists of 1089 bp encoding a polypeptide of 363 amino acids which is most closely related to the RecA proteins from species of Rhizobiaceae and Rhodospirillaceae. A recA-deficient strain of R. palustris no. 7 was obtained by gene replacement. As expected, this strain exhibited increased sensitivity to DNA-damaging agents. Transcriptional fusions of the recA promoter region to lacZ confirmed that the R. palustris no. 7 recA gene is inducible by DNA damage. Primer extension analysis of recA mRNA located the recA gene transcriptional start. A sequential deletion of the fusion plasmid was used to delimit the promoter region of the recA gene. A gel mobility shift assay demonstrated that a DNA-protein complex is formed at this promoter region. This DNA-protein complex was not formed when protein extracts from cells treated with DNA-damaging agents were used, indicating that the binding protein is a repressor. Comparison of the minimal R. palustris no. 7 recA promoter region with the recA promoter sequences from other alpha-Proteobacteria revealed the presence of the conserved sequence GAACA-N6-G(A/T)AC. Site-directed mutations that changed this consensus sequence abolished the DNA-damage-mediated expression of the R. palustris recA gene, confirming that this sequence is the SOS box of R. palustris and probably plays the same role in other alpha-Proteobacteria. | lld:pubmed |
| pubmed-article:10376844 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10376844 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376844 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10376844 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376844 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376844 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10376844 | pubmed:month | May | lld:pubmed |
| pubmed-article:10376844 | pubmed:issn | 1350-0872 | lld:pubmed |
| pubmed-article:10376844 | pubmed:author | pubmed-author:YukawaHH | lld:pubmed |
| pubmed-article:10376844 | pubmed:author | pubmed-author:DumasLL | lld:pubmed |
| pubmed-article:10376844 | pubmed:author | pubmed-author:InuiMM | lld:pubmed |
| pubmed-article:10376844 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10376844 | pubmed:volume | 145 ( Pt 5) | lld:pubmed |
| pubmed-article:10376844 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10376844 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10376844 | pubmed:pagination | 1275-85 | lld:pubmed |
| pubmed-article:10376844 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:10376844 | pubmed:meshHeading | pubmed-meshheading:10376844... | lld:pubmed |
| pubmed-article:10376844 | pubmed:meshHeading | pubmed-meshheading:10376844... | lld:pubmed |
| pubmed-article:10376844 | pubmed:meshHeading | pubmed-meshheading:10376844... | lld:pubmed |
| pubmed-article:10376844 | pubmed:meshHeading | pubmed-meshheading:10376844... | lld:pubmed |
| pubmed-article:10376844 | pubmed:meshHeading | pubmed-meshheading:10376844... | lld:pubmed |
| pubmed-article:10376844 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10376844 | pubmed:articleTitle | Molecular analysis of the recA gene and SOS box of the purple non-sulfur bacterium Rhodopseudomonas palustris no. 7. | lld:pubmed |
| pubmed-article:10376844 | pubmed:affiliation | Molecular Microbiology and Genetics Lab, Research Institute of Innovative Technology for the Earth, Kyoto, Japan. | lld:pubmed |
| pubmed-article:10376844 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10376844 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10376844 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10376844 | lld:pubmed |
