Source:http://linkedlifedata.com/resource/pubmed/id/10376844
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1999-9-21
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| pubmed:databankReference | |
| pubmed:abstractText |
The recA gene of the purple non-sulfur bacterium Rhodopseudomonas palustris no. 7 was isolated by a PCR-based method and sequenced. The complete nucleotide sequence consists of 1089 bp encoding a polypeptide of 363 amino acids which is most closely related to the RecA proteins from species of Rhizobiaceae and Rhodospirillaceae. A recA-deficient strain of R. palustris no. 7 was obtained by gene replacement. As expected, this strain exhibited increased sensitivity to DNA-damaging agents. Transcriptional fusions of the recA promoter region to lacZ confirmed that the R. palustris no. 7 recA gene is inducible by DNA damage. Primer extension analysis of recA mRNA located the recA gene transcriptional start. A sequential deletion of the fusion plasmid was used to delimit the promoter region of the recA gene. A gel mobility shift assay demonstrated that a DNA-protein complex is formed at this promoter region. This DNA-protein complex was not formed when protein extracts from cells treated with DNA-damaging agents were used, indicating that the binding protein is a repressor. Comparison of the minimal R. palustris no. 7 recA promoter region with the recA promoter sequences from other alpha-Proteobacteria revealed the presence of the conserved sequence GAACA-N6-G(A/T)AC. Site-directed mutations that changed this consensus sequence abolished the DNA-damage-mediated expression of the R. palustris recA gene, confirming that this sequence is the SOS box of R. palustris and probably plays the same role in other alpha-Proteobacteria.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1350-0872
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
145 ( Pt 5)
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1275-85
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10376844-Amino Acid Sequence,
pubmed-meshheading:10376844-Base Sequence,
pubmed-meshheading:10376844-Cloning, Molecular,
pubmed-meshheading:10376844-DNA, Bacterial,
pubmed-meshheading:10376844-DNA Mutational Analysis,
pubmed-meshheading:10376844-Gene Deletion,
pubmed-meshheading:10376844-Gene Expression Regulation, Bacterial,
pubmed-meshheading:10376844-Genes, Bacterial,
pubmed-meshheading:10376844-Molecular Sequence Data,
pubmed-meshheading:10376844-Operator Regions, Genetic,
pubmed-meshheading:10376844-Polymerase Chain Reaction,
pubmed-meshheading:10376844-Promoter Regions, Genetic,
pubmed-meshheading:10376844-Rec A Recombinases,
pubmed-meshheading:10376844-Rhodopseudomonas,
pubmed-meshheading:10376844-SOS Response (Genetics),
pubmed-meshheading:10376844-Transcription, Genetic
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| pubmed:year |
1999
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| pubmed:articleTitle |
Molecular analysis of the recA gene and SOS box of the purple non-sulfur bacterium Rhodopseudomonas palustris no. 7.
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| pubmed:affiliation |
Molecular Microbiology and Genetics Lab, Research Institute of Innovative Technology for the Earth, Kyoto, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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