Source:http://linkedlifedata.com/resource/pubmed/id/10375648
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1999-8-18
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pubmed:abstractText |
To understand the mechanism(s) underlying the Cd2+- and Co2+-induced increases in the cytosolic free Ca2+ concentration ([Ca]i) in cat adrenal chromaffin cells, we used nystatin-perforated patch recording method and fura-2 microfluorometry. Under the current-clamp conditions, the external application of 5x10(-7) M Cd2+ slowly depolarized the cells resulting in the bursting of action potentials. Under the voltage-clamp conditions, Cd2+ evoked a slow inward current accompanied by a decrease of K+ conductance at a holding potential of -40 mV, and Co2+ mimicked Cd2+ action. In some cells (16%), Cd2+ evoked an additional rapid transient outward current associated with an increased K+ conductance and a successive slow inward current. The Cd2+-induced inward current was activated in a concentration-dependent manner with a half-maximum concentration of 9.3x10(-8) M. The Cd2+- and Co2+-induced [Ca]i increases measured with fura-2 microfluorometry were maximal at 10(-6) and 10(-5) M, respectively, and the higher concentrations of both cations caused the smaller responses. Additional transient increase in [Ca]i was often evoked upon the removal of relatively higher concentrations of these metals. It was concluded that the Cd2+-induced membrane depolarization due to the decrease in K+ conductances evoked the bursting firings resulting in the increase in [Ca]i, and consequently might stimulate the catecholamine secretion.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cadmium,
http://linkedlifedata.com/resource/pubmed/chemical/Cobalt,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Ionophores,
http://linkedlifedata.com/resource/pubmed/chemical/Nystatin,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-8993
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 1999 Elsevier Science B.V.
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
832
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-30
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10375648-Animals,
pubmed-meshheading:10375648-Cadmium,
pubmed-meshheading:10375648-Cats,
pubmed-meshheading:10375648-Chromaffin Cells,
pubmed-meshheading:10375648-Cobalt,
pubmed-meshheading:10375648-Cytophotometry,
pubmed-meshheading:10375648-Enzyme Inhibitors,
pubmed-meshheading:10375648-Fluorescent Dyes,
pubmed-meshheading:10375648-Fura-2,
pubmed-meshheading:10375648-Ionophores,
pubmed-meshheading:10375648-Membrane Potentials,
pubmed-meshheading:10375648-Nystatin,
pubmed-meshheading:10375648-Patch-Clamp Techniques,
pubmed-meshheading:10375648-Thapsigargin
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pubmed:year |
1999
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pubmed:articleTitle |
Excitatory effect of Cd2+ on cat adrenal chromaffin cells.
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pubmed:affiliation |
Department of Physiology, Kagoshima University, Faculty of Medicine, Kagoshima, 890-8520, Japan. ciliary@med2.kufm.kagoshima-u.ac.jp
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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