Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1999-8-19
pubmed:abstractText
Chick/quail transplantation experiments were performed to analyse possible factors involved in the regionalisation of the midbrain-hindbrain domain. The caudal prosomeres, expressing Otx2, were transplanted at stage HH10 into rostrocaudal levels of the midbrain-hindbrain domain, either straddling the intra-metencephalic constriction (type 1 grafts), or at rostral and medial levels of pro-rhombomere A1 (type 2 and 3 grafts, respectively); thus, in all situations, one border of the graft was in contact with the host Gbx2- and Fgf8-expressing domains. The area containing the graft, recognised by QCPN immunohistochemistry, was first analysed 48 hours after transplantation for Otx2, Gbx2, En2 and Fgf8. Although in all three situations, a large part of the graft maintained Otx2 expression, another part became Otx2 negative and was induced to express Gbx2 and Fgf8. These inductive events occurred exclusively at the interface between the Otx2-positive transplanted domain and the ipsilateral host Gbx2-positive rhombomere 1, creating a new Otx2-Gbx2 boundary within the grafted territory. In type 1 and 2 grafts, the induced Fgf8 domain is in continuity with the host Fgf8 isthmic domain, whereas for type 3 grafts, these two domains are separate. High levels of En2 expression were also induced in the area expressing Gbx2 and Fgf8, and Wnt1 and Pax2 expressions, analysed in type 3 grafts, were induced at the intragraft Otx2-Gbx2 new boundary. Moreover, at later embryonic stages, the graft developed meso-isthmo-cerebellar structures. Thus, gene expressions induced in the grafted prosencephalon not only mimicked the pattern observed in the normal midbrain-hindbrain domain, but is followed by midbrain-hindbrain cytodifferentiation, indicating that not only Fgf8 but also confrontation of Otx2 and Gbx2 may play an essential role during midbrian-hindbrain regionalisation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factor 8, http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factors, http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Otx Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/PAX2 Transcription Factor, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Wnt1 Protein, http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins, http://linkedlifedata.com/resource/pubmed/chemical/engrailed 2 protein, http://linkedlifedata.com/resource/pubmed/chemical/gbx2 protein, zebrafish
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
126
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3191-203
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10375509-Animals, pubmed-meshheading:10375509-Chick Embryo, pubmed-meshheading:10375509-DNA-Binding Proteins, pubmed-meshheading:10375509-Embryonic Induction, pubmed-meshheading:10375509-Epithelium, pubmed-meshheading:10375509-Fibroblast Growth Factor 8, pubmed-meshheading:10375509-Fibroblast Growth Factors, pubmed-meshheading:10375509-Gene Expression Regulation, Developmental, pubmed-meshheading:10375509-Homeodomain Proteins, pubmed-meshheading:10375509-Mesencephalon, pubmed-meshheading:10375509-Nerve Tissue Proteins, pubmed-meshheading:10375509-Otx Transcription Factors, pubmed-meshheading:10375509-PAX2 Transcription Factor, pubmed-meshheading:10375509-Prosencephalon, pubmed-meshheading:10375509-Proto-Oncogene Proteins, pubmed-meshheading:10375509-Quail, pubmed-meshheading:10375509-Repressor Proteins, pubmed-meshheading:10375509-Rhombencephalon, pubmed-meshheading:10375509-Survival Rate, pubmed-meshheading:10375509-Trans-Activators, pubmed-meshheading:10375509-Transcription Factors, pubmed-meshheading:10375509-Transplants, pubmed-meshheading:10375509-Wnt Proteins, pubmed-meshheading:10375509-Wnt1 Protein, pubmed-meshheading:10375509-Zebrafish Proteins
pubmed:year
1999
pubmed:articleTitle
Fgf8 and Gbx2 induction concomitant with Otx2 repression is correlated with midbrain-hindbrain fate of caudal prosencephalon.
pubmed:affiliation
INSERM Unité 106, Hôpital de la Salpêtrière, Bld. de l'Hôpital, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't