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rdf:type |
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lifeskim:mentions |
umls-concept:C0031667,
umls-concept:C0037382,
umls-concept:C0042479,
umls-concept:C0205225,
umls-concept:C0205314,
umls-concept:C0206313,
umls-concept:C0678594,
umls-concept:C0679622,
umls-concept:C0936012,
umls-concept:C1521761,
umls-concept:C1880022,
umls-concept:C1998793
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pubmed:issue |
1
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pubmed:dateCreated |
1999-7-15
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pubmed:databankReference |
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pubmed:abstractText |
This paper describes the isolation and primary structure analysis of a new phospholipase A2 with platelet-aggregation-inhibiting activity from the venom of Bothrops jararaca. The protein, named BJ-PLA2, was isolated by means of ammonium sulfate precipitation and anion-exchange and reversed-phase chromatographies and behaved as a homogeneous single-chain protein on SDS-PAGE. Its amino acid sequence was determined by N-terminal sequencing and analysis of overlapped chemical and proteolytic fragments by automated Edman degradation and mass spectometry determination. BJ-PLA2 consists of 124 amino acid residues and has the structural features of snake venom class II phospholipases A2. Chemical modification with p-bromophenacylbromide caused complete loss of enzymatic activity and partially affected the platelet-aggregation-inhibiting activity of BJ-PLA2.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0003-9861
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
367
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
26-32
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10375395-Acetophenones,
pubmed-meshheading:10375395-Adenosine Diphosphate,
pubmed-meshheading:10375395-Alkylation,
pubmed-meshheading:10375395-Amino Acid Sequence,
pubmed-meshheading:10375395-Animals,
pubmed-meshheading:10375395-Binding Sites,
pubmed-meshheading:10375395-Bothrops,
pubmed-meshheading:10375395-Chemical Precipitation,
pubmed-meshheading:10375395-Chromatography, Liquid,
pubmed-meshheading:10375395-Collagen,
pubmed-meshheading:10375395-Humans,
pubmed-meshheading:10375395-Inhibitory Concentration 50,
pubmed-meshheading:10375395-Mice,
pubmed-meshheading:10375395-Molecular Sequence Data,
pubmed-meshheading:10375395-Molecular Weight,
pubmed-meshheading:10375395-Phospholipases A,
pubmed-meshheading:10375395-Phospholipases A2,
pubmed-meshheading:10375395-Platelet Aggregation,
pubmed-meshheading:10375395-Platelet Aggregation Inhibitors,
pubmed-meshheading:10375395-Sequence Homology, Amino Acid,
pubmed-meshheading:10375395-Snake Venoms
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pubmed:year |
1999
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pubmed:articleTitle |
A novel phospholipase A2, BJ-PLA2, from the venom of the snake Bothrops jararaca: purification, primary structure analysis, and its characterization as a platelet-aggregation-inhibiting factor.
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pubmed:affiliation |
Laboratory of Biochemistry and Biophysics and Laboratory of Pathophysiology Instituto Butantan, São Paulo, 05503-900, Brazil. s_serrano@hotmail.com
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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