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rdf:type |
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lifeskim:mentions |
umls-concept:C0019682,
umls-concept:C0019699,
umls-concept:C0019704,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0205245,
umls-concept:C0596988,
umls-concept:C0598312,
umls-concept:C1334043,
umls-concept:C1425322,
umls-concept:C1512032,
umls-concept:C2347961
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pubmed:issue |
6
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pubmed:dateCreated |
1999-7-1
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pubmed:abstractText |
The HIV-1 Rev protein facilitates the nuclear export of mRNA containing the Rev response element (RRE) through binding to the export receptor CRM-1. Here we show that a cellular nuclear protein, Sam68 (Src-associated protein in mitosis), specifically interacts with RRE and can partially substitute for as well as synergize with Rev in RRE-mediated gene expression and virus replication. Differential sensitivity to leptomycin B, an inhibitor of CRM-1, indicates that the export pathways mediated by Rev and Sam68 are distinct. C-terminally deleted mutants of Sam68 inhibited the transactivation of RRE-mediated expression by both wild-type Sam68 and Rev. They were retained in the cytoplasm and impeded the nuclear localization of Rev in co-expressed cells. These mutants also inhibited wild-type HIV-1 replication to the same extent as the RevM10 mutant, and may be useful as anti-viral agents in the treatment of AIDS.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Unsaturated,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, rev,
http://linkedlifedata.com/resource/pubmed/chemical/KHDRBS1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Karyopherins,
http://linkedlifedata.com/resource/pubmed/chemical/Kinetin,
http://linkedlifedata.com/resource/pubmed/chemical/Purines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/exportin 1 protein,
http://linkedlifedata.com/resource/pubmed/chemical/leptomycin B,
http://linkedlifedata.com/resource/pubmed/chemical/olomoucine,
http://linkedlifedata.com/resource/pubmed/chemical/rev Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1078-8956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
635-42
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10371501-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:10371501-Antibodies,
pubmed-meshheading:10371501-Carrier Proteins,
pubmed-meshheading:10371501-Cells, Cultured,
pubmed-meshheading:10371501-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:10371501-Cytoplasm,
pubmed-meshheading:10371501-DNA-Binding Proteins,
pubmed-meshheading:10371501-Enzyme Inhibitors,
pubmed-meshheading:10371501-Fatty Acids, Unsaturated,
pubmed-meshheading:10371501-Gene Expression Regulation, Viral,
pubmed-meshheading:10371501-Gene Products, rev,
pubmed-meshheading:10371501-Genes, Dominant,
pubmed-meshheading:10371501-Genes, Reporter,
pubmed-meshheading:10371501-HIV-1,
pubmed-meshheading:10371501-HeLa Cells,
pubmed-meshheading:10371501-Humans,
pubmed-meshheading:10371501-Karyopherins,
pubmed-meshheading:10371501-Kinetin,
pubmed-meshheading:10371501-Mutation,
pubmed-meshheading:10371501-Purines,
pubmed-meshheading:10371501-RNA-Binding Proteins,
pubmed-meshheading:10371501-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:10371501-Response Elements,
pubmed-meshheading:10371501-Virus Replication,
pubmed-meshheading:10371501-rev Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1999
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pubmed:articleTitle |
Inhibition of HIV replication by dominant negative mutants of Sam68, a functional homolog of HIV-1 Rev.
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pubmed:affiliation |
Department of Medicine, University of California San Diego, La Jolla 92093, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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