Source:http://linkedlifedata.com/resource/pubmed/id/10370058
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1999-7-7
|
| pubmed:abstractText |
Anion selectivity of the cystic fibrosis conductance transmembrane conductance regulator (CFTR) and other channels and parallel pathways expressed endogenously in apical membranes of polarized Calu-3 epithelial monolayers was studied under control conditions and during cAMP stimulation. Basolateral membranes were eliminated using alpha-toxin. The cAMP-stimulated, gradient-driven currents had the sequence Br>/=Cl>/=NO3>SCN> I>/=F>formate>HCO3>acetate>propionate=butyrate=ATP= PPi=PO4=SO4=0. Selectivity of parallel cAMP-independent pathway(s) was Br>Cl=SCN=NO3>I>formate=F >HCO3>acetate>propionate. SCN, I, F or formate blocked cAMP-stimulated, but not control, Cl currents. Anions >0.53 nm in diameter were impermeant, suggesting that the apical CFTR channel has a limiting diameter of 0.53 nm. The selectivity, blocking patterns and pore size of the cAMP-stimulated conductance pathway were very similar to those in previous reports in which CFTR was heterologously expressed in non-epithelial cells. Thus, CFTR appears to be the major apical anion conductance pathway in Calu-3 cells, and its conduction properties are independent of the expression system. CFTR in Calu-3 cells also conducts physiologically relevant anions, but not ATP, PO4 or SO4. A pathway parallel (probably a tight junction) showed a different selectivity than CFTR.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anions,
http://linkedlifedata.com/resource/pubmed/chemical/Bicarbonates,
http://linkedlifedata.com/resource/pubmed/chemical/CFTR protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cystic Fibrosis Transmembrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrates,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Propionates,
http://linkedlifedata.com/resource/pubmed/chemical/Thiocyanates,
http://linkedlifedata.com/resource/pubmed/chemical/thiocyanate
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0031-6768
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
437
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
812-22
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:10370058-Adenocarcinoma,
pubmed-meshheading:10370058-Anions,
pubmed-meshheading:10370058-Bicarbonates,
pubmed-meshheading:10370058-Cell Membrane,
pubmed-meshheading:10370058-Cell Membrane Permeability,
pubmed-meshheading:10370058-Cyclic AMP,
pubmed-meshheading:10370058-Cystic Fibrosis Transmembrane Conductance Regulator,
pubmed-meshheading:10370058-Electric Conductivity,
pubmed-meshheading:10370058-Epithelial Cells,
pubmed-meshheading:10370058-Fatty Acids,
pubmed-meshheading:10370058-Humans,
pubmed-meshheading:10370058-Lung,
pubmed-meshheading:10370058-Lung Neoplasms,
pubmed-meshheading:10370058-Nitrates,
pubmed-meshheading:10370058-Phosphates,
pubmed-meshheading:10370058-Propionates,
pubmed-meshheading:10370058-Thiocyanates,
pubmed-meshheading:10370058-Tumor Cells, Cultured
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Anion selectivity of apical membrane conductance of Calu 3 human airway epithelium.
|
| pubmed:affiliation |
Department of Molecular and Cell Biology, 231 Life Science Addition, University of California at Berkeley, Berkeley, CA 94720-3200, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|