Linked Life Data

10370015

Source:http://linkedlifedata.com/resource/pubmed/id/10370015

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1999-7-15
pubmed:abstractText
Hepatocyte transplantation using three-dimensional (3D) matrices is under evaluation as an alternative therapy for liver diseases. It is known that hepatotropic stimulation optimizes hepatocyte engraftment. We investigated hepatotrophic stimulation by portocaval shunt operation (PCS) or pancreatic islet cotransplantation (ICT) over a period of 6 months. Lewis rats served as donors and recipients, respectively. One week prior to hepatocyte implantation PCS (group A) or a sham operation (groups B-D) was performed. Four polyvinyl-alcohol matrices, each containing 1.25 x 10(7) hepatocytes (groups A and C), 1.25 x 10(7) hepatocytes and 500 islets (group B), or cell-free culture medium (group D, control) were implanted between recipients' small-bowel mesenteric leaves. One, 3, and 6 months after implantation eight polymers from each group were harvested and analyzed by morphometry, PAS reaction, and immunohistochemistry for insulin, glucagon, and bromodesoxyuridine. Morphologically healthy-appearing hepatocytes were found in all cell transplantation groups at all times. Stimulation by either PCS or ICT significantly increased hepatocyte area at 1 and 6 months compared to unstimulated specimens (group C). Over time, an increase in hepatocyte area was noted in all groups. There were no significant differences in proliferation ratios between the three experimental groups. The initially reduced PAS reaction became normal after 3 months. 3D matrices provided a sufficient environment for transplanted hepatocytes and islets. Hepatocytes proliferated and maintained differentiation independent of hepatotrophic stimulation for at least 6 months when 3D matrices were utilized. ICT efficiently stimulated transplanted hepatocytes by means of hepatocyte area. These results justify further research on hepatocyte transplantation and ICT with regard to clinical application.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0179-0358
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
168-74
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Heterotopic hepatocyte transplantation utilizing pancreatic islet cotransplantation for hepatotrophic stimulation: morphologic and morphometric evaluation.
pubmed:affiliation
Department of Pediatric Surgery, Kinderchirurgisches Forschungslabor PAV. 141, Universitätsklinikum Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't