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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1999-8-19
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pubmed:abstractText |
Intercellular Ca2+ signaling in intact salivary glands of the blowfly Calliphora erythrocephala was studied by fluorimetric digital imaging combined with microinjection of putative messenger molecules. Iontophoretic injection of D-myo-inositol 1,4, 5-trisphosphate (InsP3) into salivary gland cells evoked regenerative intercellular Ca2+ waves that spread through the impaled cell and several rows of surrounding cells. Ca2+ increases induced by microinjection of Ca2+ ions were confined to the injected cells and their nearest neighbors. Depletion of intracellular Ca2+ stores by thapsigargin pre-treatment did not alter the time course of the Ca2+ increase caused by Ca2+ injection. However, activation of Ca2+ release became clearly evident when Ca2+ was injected in the presence of serotonin (5-HT). Under these conditions, injection of Ca2+ triggered intercellular Ca2+ waves that consecutively passed through >10 cells. The phospholipase C inhibitor U73122 blocked 5-HT-induced Ca2+ increases but did not affect InsP3-dependent Ca2+ spiking and intercellular Ca2+ wave propagation. The results demonstrate that propagation of agonist-evoked Ca2+ waves in the blowfly salivary gland requires supra-basal [InsP3] but does not depend on feedback activation of phospholipase C. We conclude that the intra- and intercellular transmission of these Ca2+ waves is mediated by diffusion of Ca2+ and Ca2+-induced Ca2+ release via the InsP3 receptor channel.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1329108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1351732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1411526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1465619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1510656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1568248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1569053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1647879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1648178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1675864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1867714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1946414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1967852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-1986413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-2011747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-2017683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-2078569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-2147038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-219851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-2296584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-2304911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-2335224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-2373998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-2541501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-2784857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-3838314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-6309146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-6309155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-7631796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-7713913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-8131190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-8143927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-8274661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-8304425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-8322938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-8381210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-8580312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-8838659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-8897836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-9012354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-9097929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-9105734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-9218795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-9311999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-9547298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-9570010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10369663-9782162
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(6-((3-methoxyestra-1,3,5(10)-trie...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Estrenes,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidinones,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3222-31
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10369663-Animals,
pubmed-meshheading:10369663-Calcium,
pubmed-meshheading:10369663-Diptera,
pubmed-meshheading:10369663-Salivary Glands,
pubmed-meshheading:10369663-Serotonin,
pubmed-meshheading:10369663-Estrenes,
pubmed-meshheading:10369663-Diffusion,
pubmed-meshheading:10369663-Pyrrolidinones,
pubmed-meshheading:10369663-Serotonin Antagonists,
pubmed-meshheading:10369663-Time Factors,
pubmed-meshheading:10369663-Feedback,
pubmed-meshheading:10369663-Enzyme Activation,
pubmed-meshheading:10369663-Microinjections,
pubmed-meshheading:10369663-Calcium Signaling,
pubmed-meshheading:10369663-Type C Phospholipases,
pubmed-meshheading:10369663-Calcium Channels
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