Linked Life Data

10365897

Source:http://linkedlifedata.com/resource/pubmed/id/10365897

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-7-22
pubmed:abstractText
Maintenance of gastric mucosal integrity depends on the balance between cell loss due to apoptosis and cell proliferation. Helicobacter pylori induces apoptosis in gastric epithelial cells, but the regulation of this process has been little studied. The Bcl-2 proteins are the best-studied family of proteins involved in the mechanism of apoptotic death. Some members of this family, such as Bcl-2, inhibit apoptosis, whereas others, such as Bax, induce it. The present study was performed to determine the apoptosis rate and mRNA and protein expression for Bax and Bcl-2 in the gastric mucosa of duodenal ulcer (DU) patients with H. pylori infection before and after H. pylori eradication. We recruited 8 H. pylori-negative control subjects and 20 DU patients (H. pylori-positive) given a 1-week triple therapy to eradicate H. pylori. The apoptosis was analyzed by means of terminal deoxyribonucleotide transferase-mediated digoxigenin-11-deoxyuridine triphosphate biotin nick-end labeling (TUNEL) staining, and the expression of mRNA for Bax and Bcl-2 by reverse transcription polymerase chain reaction (RT-PCR) and Southern blot. In all patients gastritis was assessed histologically on the basis of the Sydney classification, the presence of H. pylori, and analysis of cagA status.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0036-5521
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
375-83
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10365897-Adult, pubmed-meshheading:10365897-Antigens, Bacterial, pubmed-meshheading:10365897-Apoptosis, pubmed-meshheading:10365897-Bacterial Proteins, pubmed-meshheading:10365897-Blotting, Southern, pubmed-meshheading:10365897-Blotting, Western, pubmed-meshheading:10365897-Duodenal Ulcer, pubmed-meshheading:10365897-Gastric Mucosa, pubmed-meshheading:10365897-Helicobacter Infections, pubmed-meshheading:10365897-Helicobacter pylori, pubmed-meshheading:10365897-Humans, pubmed-meshheading:10365897-Proto-Oncogene Proteins, pubmed-meshheading:10365897-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:10365897-RNA, Messenger, pubmed-meshheading:10365897-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10365897-Up-Regulation, pubmed-meshheading:10365897-bcl-2-Associated X Protein
pubmed:year
1999
pubmed:articleTitle
Helicobacter pylori induces apoptosis in gastric mucosa through an upregulation of Bax expression in humans.
pubmed:affiliation
Dept. of Internal Medicine I and Institute of Pathology, University of Erlangen-Nuremberg, Erlangen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't