Linked Life Data

10364468

Source:http://linkedlifedata.com/resource/pubmed/id/10364468

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-7-16
pubmed:abstractText
We investigated if steroid hormones regulate the secretion and the expression of leptin in female and male rat adipose tissue fragments in vitro. Dexamethasone time and dose-dependently increased the secretion and mRNA expression of leptin with a half-maximal stimulation of approximately 1 nM. A time-course revealed a maximal stimulatory effect of 17 beta-estradiol after 24 hours. In male adipose tissue 17 beta-estradiol increased leptin secretion (32% by 50 nM 17 beta-estradiol, P = 0.07 and 34% by 500 nM 17 beta-estradiol, P < 1780.05) after 24 hours. An additional effect of estrogen was seen in the dexamethasone (50 nM) stimulated cells (38% with 50 nM 17 beta-estradiol, P < 0.05 and 48% by 500 nM 17 beta-estradiol, P < 0.05). Basal secretion of leptin was equal in female and male adipose tissue, whereas the effects of 17 beta-estradiol (50 nM) and dexamethasone were significantly increased in female as compared with male adipose tissue. Progesterone, testosterone, dihydrotestosterone and dehydroepiandrostendione-sulfate neither affected leptin secretion in male nor female adipose tissue in vitro. Furthermore, to investigate the effect of estrogen female rats were ovariectomized (OVX) and the adipose tissue was incubated in vitro and compared with adipose tissue leptin secretion from sham operated rats (SHAM), and with ovariectomized rats treated with 17 beta-estradiol (EST). A decreased basal and dexamethasone-stimulated leptin secretion from OVX rats compared with SHAM rats was found (P < 0.005) whereas 17 beta-estradiol treatment of ovariectomized rats maintained a normal leptin secretion. However, the dexamethasone stimulation was equally increased above basal levels in SHAM, OVX and EST rats (3.7 +/- 1.2, 2.9 +/- 0.8, 4.2 +/- 1.4, NS, ANOVA) respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
259
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
624-30
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10364468-Adipose Tissue, pubmed-meshheading:10364468-Animals, pubmed-meshheading:10364468-Dexamethasone, pubmed-meshheading:10364468-Dose-Response Relationship, Drug, pubmed-meshheading:10364468-Estradiol, pubmed-meshheading:10364468-Female, pubmed-meshheading:10364468-Gene Expression Regulation, pubmed-meshheading:10364468-Glucocorticoids, pubmed-meshheading:10364468-Gonadal Steroid Hormones, pubmed-meshheading:10364468-Leptin, pubmed-meshheading:10364468-Male, pubmed-meshheading:10364468-Ovariectomy, pubmed-meshheading:10364468-Proteins, pubmed-meshheading:10364468-RNA, Messenger, pubmed-meshheading:10364468-Rats, pubmed-meshheading:10364468-Rats, Wistar, pubmed-meshheading:10364468-Sex Factors, pubmed-meshheading:10364468-Testosterone, pubmed-meshheading:10364468-Time Factors
pubmed:year
1999
pubmed:articleTitle
Regulation of leptin by steroid hormones in rat adipose tissue.
pubmed:affiliation
Department of Endocrinology and Metabolism, Aarhus University Hospital, Aarhus Amtssygehus, Aarhus C., DK-8000, Denmark.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't