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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1999-7-16
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pubmed:databankReference |
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pubmed:abstractText |
Transcription factors Pax-4 and Pax-6 are known to be key regulators of pancreatic cell differentiation and development. We report on the cloning of a mouse Pax-4 gene, which contains 10 exons, spanning a 4. 7-kbp region. The gene-targeting experiments revealed that Pax-4 and Pax-6 cannot substitute for each other in tissue with overlapping expression of both genes. We identified DNA-binding specificities of Pax-4 paired domain and paired-type homeodomain. Despite the different Pax-4 amino acid residues in positions responsible for Pax-6 paired-domain specificity, the DNA-binding specificities of Pax-4 and Pax-6 are similar. The Pax-4 homeodomain was shown to preferentially dimerize on DNA sequences consisting of an inverted TAAT motif, separated by 4-nucleotide spacing. The Pax-4 transactivation domain was localized within its C-terminal region, which transactivated GAL-based reporter 2.5-fold less than the C-terminal region of Pax-6. We believe that Pax-4 can act as a Pax-6 "repressor," due to the competition for binding sites and lower transactivation potential of Pax-4.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Eye Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/PAX4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PAX6 protein,
http://linkedlifedata.com/resource/pubmed/chemical/Paired Box Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Pax4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
259
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
510-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10364449-Amino Acid Sequence,
pubmed-meshheading:10364449-Animals,
pubmed-meshheading:10364449-Base Sequence,
pubmed-meshheading:10364449-Cells, Cultured,
pubmed-meshheading:10364449-Cloning, Molecular,
pubmed-meshheading:10364449-DNA-Binding Proteins,
pubmed-meshheading:10364449-Dose-Response Relationship, Drug,
pubmed-meshheading:10364449-Exons,
pubmed-meshheading:10364449-Eye Proteins,
pubmed-meshheading:10364449-Homeodomain Proteins,
pubmed-meshheading:10364449-Humans,
pubmed-meshheading:10364449-Introns,
pubmed-meshheading:10364449-Kidney,
pubmed-meshheading:10364449-Luciferases,
pubmed-meshheading:10364449-Mice,
pubmed-meshheading:10364449-Molecular Sequence Data,
pubmed-meshheading:10364449-Paired Box Transcription Factors,
pubmed-meshheading:10364449-Repressor Proteins,
pubmed-meshheading:10364449-Sequence Homology, Amino Acid,
pubmed-meshheading:10364449-Transcription Factors,
pubmed-meshheading:10364449-Transcriptional Activation
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pubmed:year |
1999
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pubmed:articleTitle |
DNA binding and transactivating properties of the paired and homeobox protein Pax4.
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pubmed:affiliation |
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic. Anna@biomed.cas.cz
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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