|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0035647,
umls-concept:C0086860,
umls-concept:C0128479,
umls-concept:C0185117,
umls-concept:C0332183,
umls-concept:C0457083,
umls-concept:C0546837,
umls-concept:C1515035,
umls-concept:C1705535,
umls-concept:C2911684
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1999-7-7
|
|
pubmed:abstractText |
We have examined the expression of midkine (MK), a neurotrophic factor with heparin-binding activity, in human esophageal cancer cells. Seven esophageal cell lines tested expressed the transcript and 8 out of 14 human esophageal tumor specimens were positively stained with anti-MK antibody, while surrounding normal esophageal tissues in these specimens were not stained. The 5'-flanking, 2.3 kb genomic region of the MK gene was shown to drive the transcription of a reporter gene in the esophageal cell lines in a cis acting manner. Forced expression in esophageal cancer cells of herpes simplex virus-thymidine kinase gene mediated by the flanking region of the MK gene conferred sensitivity to a prodrug, ganciclovir. The 5'-upstream region of the MK gene thus possesses putative promoter activity which can be used for suicide gene-based gene therapy for esophageal cancer.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0910-5050
|
|
pubmed:author |
pubmed-author:AsanoTT,
pubmed-author:IkematsuSS,
pubmed-author:KadomatsuKK,
pubmed-author:MatsubaraSS,
pubmed-author:MiyauchiMM,
pubmed-author:MuramatsuTT,
pubmed-author:OchiaiTT,
pubmed-author:SakiyamaSS,
pubmed-author:ShimadaHH,
pubmed-author:TagawaMM,
pubmed-author:TakenagaKK
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
90
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
469-75
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:10363587-Aged,
pubmed-meshheading:10363587-Antiviral Agents,
pubmed-meshheading:10363587-Carrier Proteins,
pubmed-meshheading:10363587-Cytokines,
pubmed-meshheading:10363587-Esophageal Neoplasms,
pubmed-meshheading:10363587-Female,
pubmed-meshheading:10363587-Ganciclovir,
pubmed-meshheading:10363587-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:10363587-Gene Therapy,
pubmed-meshheading:10363587-Gene Transfer Techniques,
pubmed-meshheading:10363587-Humans,
pubmed-meshheading:10363587-Immunohistochemistry,
pubmed-meshheading:10363587-Male,
pubmed-meshheading:10363587-Middle Aged,
pubmed-meshheading:10363587-Neoplasm Proteins,
pubmed-meshheading:10363587-Nerve Growth Factors,
pubmed-meshheading:10363587-Promoter Regions, Genetic,
pubmed-meshheading:10363587-Tumor Cells, Cultured
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Frequent expression of midkine gene in esophageal cancer suggests a potential usage of its promoter for suicide gene therapy.
|
|
pubmed:affiliation |
Division of Pathology, Chiba University School of Medicine.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
