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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1999-7-14
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pubmed:abstractText |
Intracytoplasmic hyaline bodies (IHBs) resemble inclusions in hepatocellular carcinoma cells, which so far have escaped further characterization. A relationship to Mallory bodies was suggested on the basis of light microscopy and filamentous ultrastructure. A hepatocellular carcinoma containing numerous IHBs was studied. Our studies revealed immunoreactivity of IHBs with the monoclonal antibodies SMI 31 and MPM-2, which recognize hyperphosphorylated epitopes present on paired helical filaments in Alzheimer's disease brains (SMI 31) or on diverse proteins hyperphosphorylated by mitotic kinases in the M-phase of the cell cycle (MPM-2). One- and two-dimensional gel electrophoresis of tumor extracts followed by immunoblotting with SMI 31 and MPM-2 antibodies revealed a major immunoreactive protein with an apparent molecular weight between 62 and 65 kd, which was resolved into several highly acidic (pH 4.5) protein components in two-dimensional gels. This protein was undetectable in non-neoplastic liver tissue. Sequence analysis identified the SMI 31 and MPM-2 immunoreactive material as p62, indicating that p62 is a major constituent of IHBs. p62 is an only recently discovered protein that is a phosphotyrosine-independent ligand of the SH2 domain of p56(lck), a member of the c-src family of cytoplasmic kinases. Moreover, p62 binds ubiquitin and may act as an adapter linking ubiquitinated species to other proteins. These features suggest a role of p62 in signal transduction and possibly also carcinogenesis. IHBs observed in the hepatocellular carcinoma cells presented are the first indications of a role of p62 in disease.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-1323908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-1370966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-1376918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-1688400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-1692099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-1701511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-2102682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-2157323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-2162578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-2837558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-3106969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-4117027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-4119691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-4172637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-4763725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-6169613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-6574461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-7504119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-7521318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-7927269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-8269084,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-8931473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-9378753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-94041,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10362795-9527487
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0002-9440
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
154
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1701-10
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10362795-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:10362795-Amino Acid Sequence,
pubmed-meshheading:10362795-Antibodies, Monoclonal,
pubmed-meshheading:10362795-Blotting, Western,
pubmed-meshheading:10362795-Carcinoma, Hepatocellular,
pubmed-meshheading:10362795-Carrier Proteins,
pubmed-meshheading:10362795-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10362795-Fatal Outcome,
pubmed-meshheading:10362795-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:10362795-Humans,
pubmed-meshheading:10362795-Hyalin,
pubmed-meshheading:10362795-Immediate-Early Proteins,
pubmed-meshheading:10362795-Immunohistochemistry,
pubmed-meshheading:10362795-Inclusion Bodies,
pubmed-meshheading:10362795-Liver Neoplasms,
pubmed-meshheading:10362795-Male,
pubmed-meshheading:10362795-Microscopy, Electron,
pubmed-meshheading:10362795-Middle Aged,
pubmed-meshheading:10362795-Molecular Sequence Data,
pubmed-meshheading:10362795-Proteins,
pubmed-meshheading:10362795-Sequence Analysis
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pubmed:year |
1999
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pubmed:articleTitle |
Analysis of intracytoplasmic hyaline bodies in a hepatocellular carcinoma. Demonstration of p62 as major constituent.
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pubmed:affiliation |
Departments of Pathology* and Surgery,double dagger University of Graz School of Medicine, Graz, Austria.
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pubmed:publicationType |
Journal Article,
Case Reports,
Research Support, Non-U.S. Gov't
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