Linked Life Data

10362552

Source:http://linkedlifedata.com/resource/pubmed/id/10362552

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1999-9-2
pubmed:abstractText
Apoptosis is induced in various tumor cell lines by vector-dependent overexpression of the conserved gene C1D that encodes a DNA-binding and DNA-PK-activating protein. C1D is physiologically expressed in 50 human tissues tested, which points to its basic cellular function. The expression of this gene must be tightly regulated because elevated levels of C1D protein, e.g. those induced by transient vector-dependent expression, result in apoptotic cell death. Cells transfected with C1D-expressing constructs show terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling of DNA ends. Transfections with constructs in which C1D is expressed in fusion with the (enhanced) green fluorescent protein from A. victoria (EGFP) allow the transfected cells to be identified and the morphological changes induced to be traced. Starting from intense nuclear spots, green fluorescence reflecting C1D expression increases dramatically at 12-24 hours post-transfection. Expression of C1D-EGFP protein is accompanied by morphological changes typical of apoptotic cell death, e.g. cytoplasmic vacuolation, membrane blebbing and nuclear disintegration. Cell shrinkage and detachment from extracellular matrix are observed in monolayer cultures while suspension cells become progressively flattened. The facility to differentiate between transfected and non-transfected cells reveals that non-transfected cells co-cultured with transfected cells also show the morphological changes of apoptosis, which points to a bystander effect. C1D-dependent apoptosis is not induced in cells with non-functional p53. Accordingly, C1D-induced apoptosis is discussed in relation to its potential to activate DNA-PK, which has been considered to act as an upstream activator of p53.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
112 ( Pt 13)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2223-32
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:10362552-Animals, pubmed-meshheading:10362552-Apoptosis, pubmed-meshheading:10362552-Carcinoma, Ehrlich Tumor, pubmed-meshheading:10362552-Co-Repressor Proteins, pubmed-meshheading:10362552-DNA-Activated Protein Kinase, pubmed-meshheading:10362552-DNA-Binding Proteins, pubmed-meshheading:10362552-Enzyme Activation, pubmed-meshheading:10362552-Gene Expression, pubmed-meshheading:10362552-HeLa Cells, pubmed-meshheading:10362552-Humans, pubmed-meshheading:10362552-Mice, pubmed-meshheading:10362552-Nuclear Proteins, pubmed-meshheading:10362552-Plasmids, pubmed-meshheading:10362552-Protein-Serine-Threonine Kinases, pubmed-meshheading:10362552-Recombinant Fusion Proteins, pubmed-meshheading:10362552-Repressor Proteins, pubmed-meshheading:10362552-Transfection, pubmed-meshheading:10362552-Tumor Cells, Cultured, pubmed-meshheading:10362552-Tumor Suppressor Protein p53
pubmed:year
1999
pubmed:articleTitle
Induction of apoptosis by overexpression of the DNA-binding and DNA-PK-activating protein C1D.
pubmed:affiliation
Division Biochemistry of the Cell and Biomedical Structure Analysis Group, German Cancer Research Center, D-69120 Heidelberg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't