Linked Life Data

10362012

Source:http://linkedlifedata.com/resource/pubmed/id/10362012

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-6-17
pubmed:abstractText
Myostatin is a secreted growth and differentiating factor (GDF-8) that belongs to the transforming growth factor-beta (TGF-beta) superfamily. Targeted disruption of the myostatin gene in mice and a mutation in the third exon of the myostatin gene in double-muscled Belgian Blue cattle breed result in skeletal muscle hyperplasia. Hence, myostatin has been shown to be involved in the regulation of skeletal muscle mass in both mice and cattle. Previous published reports utilizing Northern hybridization had shown that myostatin expression was seen exclusively in skeletal muscle. A significantly lower level of myostatin mRNA was also reported in adipose tissue. Using a sensitive reverse transcription-polymerase chain reaction (RT-PCR) technique and Western blotting with anti-myostatin antibodies, we show that myostatin mRNA and protein are not restricted to skeletal muscle. We also show that myostatin expression is detected in the muscle of both fetal and adult hearts. Sequence analysis reveals that the Belgian Blue heart myostatin cDNA sequence contains an 11 nucleotide deletion in the third exon that causes a frameshift that eliminates virtually all of the mature, active region of the protein. Anti-myostatin immunostaining on heart sections also demonstrates that myostatin protein is localized in Purkinje fibers and cardiomyocytes in heart tissue. Furthermore, following myocardial infarction, myostatin expression is upregulated in the cardiomyocytes surrounding the infarct area. Given that myostatin is expressed in fetal and adult hearts and that myostatin expression is upregulated in cardiomyocytes after the infarction, myostatin could play an important role in cardiac development and physiology.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9541
pubmed:author
pubmed:issnType
Print
pubmed:volume
180
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10362012-Animals, pubmed-meshheading:10362012-Base Sequence, pubmed-meshheading:10362012-Blotting, Western, pubmed-meshheading:10362012-Cattle, pubmed-meshheading:10362012-Conserved Sequence, pubmed-meshheading:10362012-DNA, Complementary, pubmed-meshheading:10362012-Disease Models, Animal, pubmed-meshheading:10362012-Gene Expression Regulation, Developmental, pubmed-meshheading:10362012-Mammals, pubmed-meshheading:10362012-Molecular Sequence Data, pubmed-meshheading:10362012-Muscle, Skeletal, pubmed-meshheading:10362012-Muscle Fibers, Skeletal, pubmed-meshheading:10362012-Mutation, pubmed-meshheading:10362012-Myocardial Infarction, pubmed-meshheading:10362012-Myocardium, pubmed-meshheading:10362012-Myostatin, pubmed-meshheading:10362012-RNA, Messenger, pubmed-meshheading:10362012-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10362012-Sequence Homology, Amino Acid, pubmed-meshheading:10362012-Sheep, pubmed-meshheading:10362012-Transforming Growth Factor beta, pubmed-meshheading:10362012-Up-Regulation
pubmed:year
1999
pubmed:articleTitle
Myostatin, a transforming growth factor-beta superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct.
pubmed:affiliation
Growth Physiology, AgResearch, Ruakura, Hamilton, New Zealand. SharmaM@agresearch.cri.nz
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't