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rdf:type |
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lifeskim:mentions |
umls-concept:C0007613,
umls-concept:C0021289,
umls-concept:C0039194,
umls-concept:C0086418,
umls-concept:C0205216,
umls-concept:C0205225,
umls-concept:C0439831,
umls-concept:C0439836,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
3
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pubmed:dateCreated |
1999-6-23
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pubmed:abstractText |
The reduced incidence of graft versus host disease following the use of human cord blood as a source of stem cells for bone marrow reconstitution challenges our understanding of the immunocompetence of newborn T cells. Newborn CD4+ T cells express mainly the CD45RA phenotype and have been considered to respond comparably to adult CD4+ T cells exhibiting the CD45RA phenotype. We compared the in vitro kinetics of phenotypic conversion of newborn and adult CD4+CD45RA+ T cells to CD4+CD45RO+ T cells. The cytokine profile and B cell helper activity of the converted CD4+CD45RO+ T cell population were also determined. Newborn CD4+CD45RA+ T cells were converted to CD4+CD45RO+ with significantly faster time kinetics than adult CD4+CD45RA+ T cells, following either phytohaemagglutinin (PHA) or anti-CD2 activation. Freshly purified newborn naive T cells did not produce IL-2, IL-4 or interferon-gamma (IFN-gamma) following stimulation, whereas adult naive T cells secreted IL-2 and adult-derived CD4+CD45RO+ T cells secreted all three cytokines under the same stimulatory conditions. However, newborn and adult CD4+CD45RA+ T cells, following primary stimulation and maturation in vitro, acquired the ability to secrete a Th1-type cytokine profile of IL-2 and IFN-gamma after secondary stimulation. Newborn CD4+ naive T cells that acquired the CD45RO phenotype in vitro also gained B cell helper activity equivalent to that of adult in vitro matured CD4+ naive T cells. These findings suggest that newborn and adult CD4+CD45RA+ T cell subsets are differentially responsive to various stimuli. They show that newborn CD4+CD45RA+ naive T cells can transform more quickly than their adult counterparts into functionally equivalent CD4+CD45RO+ T cells, a process that may be important to counteract the immature immune environment which exists in the newborn.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-1532395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-1532537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-1557612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-1627249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-1671403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-1694200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-1696528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-1824631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-1825090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-1829508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-1978253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-1999771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-2139363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-2144907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-2410496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-2456868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-2564856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-2571931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-2965180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-2970504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-2978373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-2981090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-3919982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-3925895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-4600315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-7749122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-8100083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-8118883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-8977281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10361246-9697980
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
116
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
527-33
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10361246-Adult,
pubmed-meshheading:10361246-Antigens, CD45,
pubmed-meshheading:10361246-B-Lymphocytes,
pubmed-meshheading:10361246-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10361246-Cytokines,
pubmed-meshheading:10361246-Fetal Blood,
pubmed-meshheading:10361246-Humans,
pubmed-meshheading:10361246-Infant, Newborn,
pubmed-meshheading:10361246-Kinetics,
pubmed-meshheading:10361246-Phenotype,
pubmed-meshheading:10361246-Th1 Cells
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pubmed:year |
1999
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pubmed:articleTitle |
Rapid conversion of naive to effector T cell function counteracts diminished primary human newborn T cell responses.
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pubmed:affiliation |
Children's Research Centre, Our Lady's Hospital For Sick Children, Crumlin, Dublin, Ireland.
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pubmed:publicationType |
Journal Article,
In Vitro
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