Source:http://linkedlifedata.com/resource/pubmed/id/10361116
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
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| pubmed:dateCreated |
1999-7-1
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| pubmed:abstractText |
Earlier, we described a stromal cell-free two-step clonal culture system in which murine primitive lymphohematopoietic progenitors produce myeloid and B-lymphoid lineage cells. In the same culture T-cell potential of the progenitors was maintained. We now report that, in addition to myeloid and B-lymphoid cells, putative T-cell progenitors are also produced in culture. Lineage-negative (Lin-) Ly-6A/E+ c-kit+ bone marrow cells from 5-fluorouracil-treated mice were cultured in methylcellulose in the presence of SF (Steel factor), interleukin (IL)-11, and IL-7, and the resulting primary colonies were picked and pooled. When injected into severe combined immune deficiency (scid) mice, the pooled cells reconstituted the T-cell compartment of the scid mice earlier than freshly prepared primitive marrow cells. This reconstitution activity of the pooled primary colony cells was enriched in the Ly-6A/E+ and FcgammaRII/III-/low cell fractions. Reverse transcriptase-polymerase chain reaction (RT-PCR) and DNA-PCR analyses showed that some of the primary colony cells are differentiated sufficiently to express messenger RNA (mRNA) of T-cell receptor (TCR) beta-chain and pre-TCR alpha (pTalpha) and, although not frequently, to perform Dbeta-Jbeta rearrangement of the TCR gene. Micromanipulation studies confirmed the clonal origin of myeloid lineage cells and the cells positive for the T-cell-specific transcripts and D-J rearrangement of TCR beta-chain. These results suggested that, in the presence of SF, IL-11, and IL-7, primitive lymphohematopoietic progenitors differentiate toward T-cell lineage in addition to myeloid and B-cell lineages.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Fluorouracil,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-11,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-7,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgG,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-4971
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
93
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4187-95
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:10361116-Animals,
pubmed-meshheading:10361116-B-Lymphocytes,
pubmed-meshheading:10361116-Bone Marrow Cells,
pubmed-meshheading:10361116-Cell Differentiation,
pubmed-meshheading:10361116-Cells, Cultured,
pubmed-meshheading:10361116-Fluorouracil,
pubmed-meshheading:10361116-Granulocytes,
pubmed-meshheading:10361116-Hematopoietic Stem Cells,
pubmed-meshheading:10361116-Interleukin-11,
pubmed-meshheading:10361116-Interleukin-7,
pubmed-meshheading:10361116-Mice,
pubmed-meshheading:10361116-Mice, SCID,
pubmed-meshheading:10361116-RNA, Messenger,
pubmed-meshheading:10361116-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:10361116-Receptors, IgG,
pubmed-meshheading:10361116-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10361116-Stem Cell Factor,
pubmed-meshheading:10361116-T-Lymphocytes
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| pubmed:year |
1999
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| pubmed:articleTitle |
Differentiation in culture of murine primitive lymphohematopoietic progenitors toward T-cell lineage.
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| pubmed:affiliation |
Hokkaido Red Cross Blood Center, Sapporo, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|