Linked Life Data

10361115

Source:http://linkedlifedata.com/resource/pubmed/id/10361115

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1999-7-1
pubmed:abstractText
The mi locus encodes a member of the basic-helix-loop-helix-leucine zipper protein family of transcription factors (hereafter called MITF). Mutant alleles of mi, Mior, and Miwh are deletion or point mutation of the basic domain by which MITF binds DNA. The basic domain also has nuclear localization potential. In the present study, we compared the mast cell abnormalities of Mior/Mior and Miwh/Miwh mice with those of mi/mi mice, of which many have been described by us. The number of mast cells in the skin of Mior/Mior suckling mice was remarkably decreased from that observed in mi/mi suckling mice, but the number was normal in the skin of Miwh/Miwh suckling mice. The decrease in skin mast cells was more severe in the mi/mi embryos than in mi/mi suckling mice, but the magnitude of the decrease was comparable between Mior/Mior embryos and Mior/Mior suckling mice. The poor mRNA expression of granzyme B and tryptophan hydroxylase genes was observed in all cultured mast cells (CMCs) derived from the spleens of Miwh/Miwh, Mior/Mior, and mi/mi mice. However, the poor expression of mouse mast cell protease-4 (MMCP-4), MMCP-5, and MMCP-6 was observed only in Mior/Mior and mi/mi CMCs. MITF encoded by Miwh mutant allele (Miwh-MITF) showed deficient but demonstratable DNA binding, but mi-MITF and Mior-MITF did not show any DNA binding ability. Although Miwh-MITF and Mior-MITF showed normal nuclear localization potential, the potential was significantly impaired in mi-MITF. The rank order of mast cell abnormality (mi/mi > Mior/Mior > Miwh/Miwh) appears to be related to the functional abnormality of MITF encoded by each mutant gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4179-86
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10361115-Alleles, pubmed-meshheading:10361115-Animals, pubmed-meshheading:10361115-Animals, Suckling, pubmed-meshheading:10361115-Cells, Cultured, pubmed-meshheading:10361115-DNA, pubmed-meshheading:10361115-DNA-Binding Proteins, pubmed-meshheading:10361115-Helix-Loop-Helix Motifs, pubmed-meshheading:10361115-In Situ Hybridization, pubmed-meshheading:10361115-Leucine Zippers, pubmed-meshheading:10361115-Mast Cells, pubmed-meshheading:10361115-Mice, pubmed-meshheading:10361115-Mice, Inbred C57BL, pubmed-meshheading:10361115-Microphthalmia-Associated Transcription Factor, pubmed-meshheading:10361115-Mutation, pubmed-meshheading:10361115-Phenotype, pubmed-meshheading:10361115-Serine Endopeptidases, pubmed-meshheading:10361115-Skin, pubmed-meshheading:10361115-Transcription Factors, pubmed-meshheading:10361115-Tryptases
pubmed:year
1999
pubmed:articleTitle
Different effect of various mutant MITF encoded by mi, Mior, or Miwh allele on phenotype of murine mast cells.
pubmed:affiliation
Department of Pathology, Osaka University Medical School, Osaka, Japan; the Department of Pharmacology, Ehime University Medical School, Ehime, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't