Linked Life Data

10359845

Source:http://linkedlifedata.com/resource/pubmed/id/10359845

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1999-7-8
pubmed:databankReference
pubmed:abstractText
The uptake of iron in plants is a highly regulated process that is induced on iron starvation. In tomato, the mutant chloronerva exhibits constitutive expression of iron uptake responses and intercostal chlorosis. Biochemically, chloronerva is an auxotroph for nicotianamine, a key polyamine in plant iron uptake metabolism. The chloronerva gene has been fine-mapped onto the long arm of chromosome 1 in a large segregating tomato population and yeast artificial chromosome clones encompassing the region were isolated by using flanking markers. A cosmid contig containing the chloronerva gene was established, and complementing cosmids were identified by transformation into the mutant. The chloronerva transcript was identified by cDNA isolation using the complementing cosmids. The gene encodes a unique protein of 35 kDa. The mutant harbors a single base change compared with the wild type. Based on enzyme activity and sequence similarity to the coding DNA sequence of the purified barley enzyme the chloronerva gene encodes the enzyme nicotianamine synthase.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7098-103
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Map-based cloning of chloronerva, a gene involved in iron uptake of higher plants encoding nicotianamine synthase.
pubmed:affiliation
Institute for Plant Genetics and Crop Plant Research, Corrensstr. 3, D-06466 Gatersleben, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't