Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1999-7-8
pubmed:abstractText
Microbial populations under nonlethal selection can give rise to mutations that relieve the selective pressure, a phenomenon that has come to be called "adaptive mutation." One explanation for adaptive mutation is that a small proportion of the cells experience a period of transient hypermutation, and that these hypermutators account for the mutations that appear. The experiments reported here investigated the contribution that hypermutators make to the mutations occurring in a Lac- strain of Escherichia coli during selection for lactose utilization. A broad mutational screen, loss of motility, was used to compare the frequency of nonselected mutations in starved Lac- cells, in Lac+ revertants, and in Lac+ revertants carrying yet another nonselected mutation. These frequencies allowed us to calculate that the hypermutating subpopulation makes up approximately 0.06% of the population and that its mutation rate is elevated approximately 200-fold. From these numbers we conclude that the hypermutators are responsible for nearly all multiple mutations but produce only approximately 10% of the adaptive Lac+ mutations.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-1516815, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-1538699, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-1752431, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-1916241, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-2191182, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-2227388, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-3045565, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-4603159, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-609095, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-7040679, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-7644519, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-7777535, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-7828809, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-8257106, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-8636043, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-8692687, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-8770582, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-8811176, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-8849879, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-8990293, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-9045812, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-9192894, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-9214645, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-9278503, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-9308969, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-9560363, http://linkedlifedata.com/resource/pubmed/commentcorrection/10359804-9804552
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6862-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
The role of transient hypermutators in adaptive mutation in Escherichia coli.
pubmed:affiliation
Department of Environmental Health, Boston University School of Public Health, Boston University School of Medicine, Boston, MA 02118, USA.
pubmed:publicationType
Journal Article