Linked Life Data

10359103

Source:http://linkedlifedata.com/resource/pubmed/id/10359103

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-6-30
pubmed:abstractText
IL-5, known to be produced by T lymphocytes and eosinophils, is a key regulator of intestinal diseases such as parasitosis or eosinophilic gastroenteritis. Here we examined if mast cells contribute to the IL-5 production in human intestinal mucosa. The number of IL-5-positive lamina propria cells was substantially higher in patients with intestinal inflammatory diseases (5.3 +/- 4.6%, n = 17) compared to healthy controls (0.5 +/- 0.9%, n = 8, p < 0.01). In patients, the IL-5-positive cells were eosinophils (70 +/- 13%) and mast cells (29 +/- 14%), whereas in controls all IL-5-positive cells were eosinophils. IL-5-positive T cells were not detected, likely because they do not store IL-5. In vitro studies with isolated human intestinal mast cells and eosinophils showed that mast cells do not produce IL-5 constitutively, but release high amounts of IL-5 (315 +/- 115 pg/10(6) cells) following IgE receptor cross-linking, compared to activated eosinophils (24 +/- 5 pg/10(6) cells). Inhibitor studies suggest a regulation of IL-5 production at the transcriptional level. In conclusion our data demonstrate that activated mast cells are a potent source of IL-5 in the human intestinal mucosa.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1496-503
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Human intestinal mast cells produce IL-5 in vitro upon IgE receptor cross-linking and in vivo in the course of intestinal inflammatory disease.
pubmed:affiliation
Department of Gastroenterology and Hepatology, Medical School of Hannover, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't