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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
5
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pubmed:dateCreated |
1999-6-24
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pubmed:abstractText |
Fifty-eight patients with chronic hepatitis B (HB) or C (HC) were treated with recombinant human interferon (rIFN)-alpha 2 and their sera were assayed for antibodies to rIFN-alpha 2c. Twelve of these patients produced low titres and two high titres of the antibodies. We localized the region which was recognised by the high-titre therapy-induced antibodies on the IFN molecule by testing the antibodies with a set of murine monoclonal antibodies (MoAbs) to IFN-alpha 2 in a competitive radioimmune assay (RIA). Only MoAbs with epitopes located in the amino-terminal portion of IFN-alpha 2 could inhibit the binding of radiolabelled IFN-alpha 2 by patients' sera. Our data indicate that the therapy-induced antibodies were directed to the receptor-binding domain of IFN-alpha 2 formed by amino acids (aa) 30-53. In accordance with this observation, human anti-IFN sera inhibited the binding of rIFN-alpha 2 to human cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0001-723X
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
279-84
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10358727-Adult,
pubmed-meshheading:10358727-Aged,
pubmed-meshheading:10358727-Animals,
pubmed-meshheading:10358727-Antibody Specificity,
pubmed-meshheading:10358727-Antiviral Agents,
pubmed-meshheading:10358727-Binding Sites,
pubmed-meshheading:10358727-Epitopes,
pubmed-meshheading:10358727-Female,
pubmed-meshheading:10358727-HL-60 Cells,
pubmed-meshheading:10358727-Hepatitis B, Chronic,
pubmed-meshheading:10358727-Hepatitis C, Chronic,
pubmed-meshheading:10358727-Humans,
pubmed-meshheading:10358727-Immunoglobulin G,
pubmed-meshheading:10358727-Interferon Type I,
pubmed-meshheading:10358727-Mice,
pubmed-meshheading:10358727-Radioimmunoassay,
pubmed-meshheading:10358727-Recombinant Proteins,
pubmed-meshheading:10358727-Time Factors
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pubmed:year |
1998
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|
pubmed:articleTitle |
Therapy-induced antibodies to interferon-alpha 2a recognise its receptor-binding site.
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pubmed:affiliation |
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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