Source:http://linkedlifedata.com/resource/pubmed/id/10358167
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1999-6-30
|
| pubmed:abstractText |
Spontaneous colitis resembling ulcerative colitis developed in 3 of 10 independent TCR transgenic (Tg) mouse lines maintained under specific pathogen-free conditions. All three susceptible lines were CD4 lymphopenic, whereas resistant lines had normal numbers of CD4+ T cells. Thus, cytochrome c-specific 5C.C7 TCR Tg mice developed colitis only when crossed onto a SCID- or Rag-1-deficient background. A second line of lymphopenic cytochrome c-specific Tg mice bearing the AND TCR also developed colitis. In both cases, CD4+ T cells expressing the Tg-encoded TCR were preferentially activated in inflamed colons compared with lymph nodes or spleens. In contrast, Tg+CD4+ T cells remained quiescent in both inflamed and unaffected colons in another line of susceptible Tg mice carrying a TCR specific for myelin basic protein, suggesting a fortuitous cross-reactivity of the IEk-restricted cytochrome c-reactive AND and 5C.C7 TCRs with an Ag present in the gut. The percentage of CD4+ T cells expressing only endogenous TCR alpha-chains was increased consistently in inflamed colons in AND as well as 5C.C7 Rag-1-/- TCR Tg mice, suggesting that polyclonal CD4+ T cells were also involved in the pathogenesis of spontaneous colitis. Moreover, our data indicate that some alpha-chain rearrangement was still occurring in TCR Tg mice on a Rag-1-/- background, since activated CD4+ T cells expressing endogenously rearranged alpha-chains paired with the Tg-encoded beta-chain were detected consistently in the colons of such mice.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
162
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
7208-16
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:10358167-Adoptive Transfer,
pubmed-meshheading:10358167-Animals,
pubmed-meshheading:10358167-Antigens, CD4,
pubmed-meshheading:10358167-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10358167-Colitis, Ulcerative,
pubmed-meshheading:10358167-Disease Models, Animal,
pubmed-meshheading:10358167-Genes, RAG-1,
pubmed-meshheading:10358167-Genetic Predisposition to Disease,
pubmed-meshheading:10358167-Homeodomain Proteins,
pubmed-meshheading:10358167-Immunophenotyping,
pubmed-meshheading:10358167-Intestinal Mucosa,
pubmed-meshheading:10358167-Lymphopenia,
pubmed-meshheading:10358167-Mice,
pubmed-meshheading:10358167-Mice, Inbred C57BL,
pubmed-meshheading:10358167-Mice, SCID,
pubmed-meshheading:10358167-Mice, Transgenic,
pubmed-meshheading:10358167-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:10358167-T-Lymphocyte Subsets
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
TCR-mediated involvement of CD4+ transgenic T cells in spontaneous inflammatory bowel disease in lymphopenic mice.
|
| pubmed:affiliation |
Centenary Institute of Cancer Medicine and Cell Biology, Newtown, Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|