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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0009013,
umls-concept:C0025260,
umls-concept:C0039194,
umls-concept:C0039195,
umls-concept:C0085358,
umls-concept:C0086418,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1515655,
umls-concept:C1706438,
umls-concept:C2698600
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pubmed:issue |
12
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pubmed:dateCreated |
1999-6-30
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pubmed:databankReference |
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pubmed:abstractText |
It has been generally believed that human CD8+ memory cells are principally found within the CD45ROhigh population. There are high frequencies of CD8+ memory CTL specific for the human CMV tegument phosphoprotein pp65 in PBMC of long-term virus carriers; the large population of memory CTL specific for a given pp65 peptide contains individual CTL clones that have greatly expanded. In this study, we found high frequencies of pp65 peptide-specific memory CTL precursors in the CD45ROhighCD45RA- population, but also appreciable frequencies in the CD45RAhigh subpopulation. Because the majority of CD8+ T cells in PBMC are CD45RAhigh, more of the total pp65-specific memory CTL pool is within the CD45RAhigh than in the CD45ROhigh compartment. Using clonotypic oligonucleotide probes to quantify the size of individual pp65-specific CTL clones in vivo, we found the CD45RAhigh population contributed 6- to 10-fold more than the CD45ROhigh population to the total virus-specific clone size in CD8+ cells. During primary CMV infection, an individual virus-specific CTL clone was initially CD45ROhigh, but after resolution of infection this clone was detected in both the CD45ROhigh and the CD45RAhigh populations. We conclude that CD45RA+ human CD8+ T cells do not solely comprise naive cells, but contain a very significant proportion of memory cells, which can revert from the CD45ROhigh to CD45RAhigh phenotype in vivo.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7080-7
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pubmed:dateRevised |
2010-8-25
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pubmed:meshHeading |
pubmed-meshheading:10358151-Antigens, CD45,
pubmed-meshheading:10358151-Carrier State,
pubmed-meshheading:10358151-Cell Line,
pubmed-meshheading:10358151-Clone Cells,
pubmed-meshheading:10358151-Cytomegalovirus,
pubmed-meshheading:10358151-Cytomegalovirus Infections,
pubmed-meshheading:10358151-Cytotoxicity, Immunologic,
pubmed-meshheading:10358151-Epitopes, T-Lymphocyte,
pubmed-meshheading:10358151-Humans,
pubmed-meshheading:10358151-Immunologic Memory,
pubmed-meshheading:10358151-Leukocytes, Mononuclear,
pubmed-meshheading:10358151-Longitudinal Studies,
pubmed-meshheading:10358151-Lymphocyte Activation,
pubmed-meshheading:10358151-Molecular Sequence Data,
pubmed-meshheading:10358151-Peptides,
pubmed-meshheading:10358151-Phosphoproteins,
pubmed-meshheading:10358151-Stem Cells,
pubmed-meshheading:10358151-T-Lymphocyte Subsets,
pubmed-meshheading:10358151-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:10358151-Time Factors,
pubmed-meshheading:10358151-Viral Matrix Proteins
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pubmed:year |
1999
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pubmed:articleTitle |
Human virus-specific CD8+ CTL clones revert from CD45ROhigh to CD45RAhigh in vivo: CD45RAhighCD8+ T cells comprise both naive and memory cells.
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pubmed:affiliation |
Department of Medicine, University of Cambridge Clinical School, United Kingdom. mrw1004@mole.bio.cam.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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