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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
1999-7-6
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pubmed:abstractText |
In this paper we show that transcription factors Ets-1 and Ets-2 recruit transcription adapter proteins p300 and CBP (cAMP-responsive element-binding protein) during the transcriptional activation of the human stromelysin promoter, which contains palindromic Ets-binding sites. Ets-2 and p300/CBP exist as a complex in vivo. Two regions of p300/CBP between amino acids (a.a.) 328 and 596 and a. a. 1678 and 2370 independently can interact with Ets-1 and Ets-2 in vitro and in vivo. Both these regions of p300/CBP bind to the transactivation domain of Ets-2, whereas the C-terminal region binds only to the DNA binding domain of Ets-2. The N- and the C-terminal regions of CBP (a.a. 1-1097 and 1678-2442, respectively) which lack histone acetylation activity independently are capable of coactivating Ets-2. Other Ets family transcription factors failed to cooperate with p300/CBP in stimulating the stromelysin promoter. The LXXLL sequence, reported to be important in receptor-coactivator interactions, does not appear to play a role in the interaction of Ets-2 with p300/CBP. Previous studies have shown that the stimulation of transcriptional activation activity of Ets-2 requires phosphorylation of threonine 72 by the Ras/mitogen-activated protein kinase signaling pathway. We show that mutation of this site does not affect its capacity to bind to and to cooperate with p300/CBP.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CREB-Binding Protein,
http://linkedlifedata.com/resource/pubmed/chemical/CREBBP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ERF protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/ERG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/ETS1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/ETS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Histone Acetyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/NCOA3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Receptor Coactivator 3,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Protein c-ets-1,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Protein c-ets-2,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-ets,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/transcription factor PEA3
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:ButticeGG,
pubmed-author:DugganCC,
pubmed-author:FassR JRJ,
pubmed-author:FerreiraEE,
pubmed-author:HausenGG,
pubmed-author:JayaramanGG,
pubmed-author:KurkinenMM,
pubmed-author:SomasundaramKK,
pubmed-author:SrinivasRR,
pubmed-author:SwaminathanSS,
pubmed-author:ThimmapayaBB,
pubmed-author:WeitzmanSS,
pubmed-author:WilliamsJJ
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
17342-52
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10358095-Binding Sites,
pubmed-meshheading:10358095-CREB-Binding Protein,
pubmed-meshheading:10358095-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:10358095-DNA-Binding Proteins,
pubmed-meshheading:10358095-Histone Acetyltransferases,
pubmed-meshheading:10358095-Humans,
pubmed-meshheading:10358095-Matrix Metalloproteinase 3,
pubmed-meshheading:10358095-Mutation,
pubmed-meshheading:10358095-Nuclear Proteins,
pubmed-meshheading:10358095-Nuclear Receptor Coactivator 3,
pubmed-meshheading:10358095-Precipitin Tests,
pubmed-meshheading:10358095-Promoter Regions, Genetic,
pubmed-meshheading:10358095-Protein Binding,
pubmed-meshheading:10358095-Proto-Oncogene Protein c-ets-1,
pubmed-meshheading:10358095-Proto-Oncogene Protein c-ets-2,
pubmed-meshheading:10358095-Proto-Oncogene Proteins,
pubmed-meshheading:10358095-Proto-Oncogene Proteins c-ets,
pubmed-meshheading:10358095-Repressor Proteins,
pubmed-meshheading:10358095-Trans-Activators,
pubmed-meshheading:10358095-Transcription Factors,
pubmed-meshheading:10358095-Transcriptional Activation
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pubmed:year |
1999
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pubmed:articleTitle |
p300/cAMP-responsive element-binding protein interactions with ets-1 and ets-2 in the transcriptional activation of the human stromelysin promoter.
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pubmed:affiliation |
Lurie Cancer Center and Microbiology and Immunology Department, Northwestern University Medical School, Chicago, Illinois 60611, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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