rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
4
|
pubmed:dateCreated |
1999-6-29
|
pubmed:abstractText |
A mistletoe lectin was isolated from water extracts of Korean mistletoe, a subspecies of Viscum album, grown on Quercus mongolica using CM-Sepharose chromatography followed by an affinity chromatography on a concanavalin A-Sepharose column. The compound proved to be a mistletoe lectin II with D-galactose and N-acetyl-D-galactosamine specificity. Matrix-assisted laser desorption time-of-flight mass spectroscopy showed it to have an average molecular mass of 62.7 kDa and to consist of two subunits of 30.6 kDa and 32.5 kDa. It was a basic protein with isoelectric points of 9.4 and 9.6 by capillary isoelectric focusing and was cytotoxic to Molt4 cell.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
1420-682X
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
55
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
679-82
|
pubmed:dateRevised |
2007-11-15
|
pubmed:meshHeading |
pubmed-meshheading:10357236-Humans,
pubmed-meshheading:10357236-Korea,
pubmed-meshheading:10357236-Lectins,
pubmed-meshheading:10357236-Mistletoe,
pubmed-meshheading:10357236-Plant Lectins,
pubmed-meshheading:10357236-Plant Preparations,
pubmed-meshheading:10357236-Plant Proteins,
pubmed-meshheading:10357236-Plants, Medicinal,
pubmed-meshheading:10357236-Ribosome Inactivating Proteins, Type 2,
pubmed-meshheading:10357236-Toxins, Biological,
pubmed-meshheading:10357236-Tumor Cells, Cultured
|
pubmed:year |
1999
|
pubmed:articleTitle |
Isolation and characterization of biologically active lectin from Korean mistletoe, Viscum album var. Coloratum.
|
pubmed:affiliation |
College of Pharmacy, Wonkwang University, Iksan, Korea. hslee@wonnms.wonkwang.ac.kr
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|