|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
24
|
|
pubmed:dateCreated |
1979-3-24
|
|
pubmed:abstractText |
Antibodies were prepared against 18 tryptic and cyanogen bromide peptides from beta-galactosidase ranging in size from 15 to 96 amino acid residues representing more than 80% of the polypeptide chain. They were tested for binding capacity and affinity toward their homologous antigens and toward the whole native protein. Nine antisera bound to beta-galactosidase; these had been raised against certain peptides from the central and carboxyl-terminal regions of the poly-peptide chain. Based on these results a preliminary model of the three-dimensional structure of the folded protein is suggested.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0006-2960
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
28
|
|
pubmed:volume |
17
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
5156-60
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:103569-Antibodies,
pubmed-meshheading:103569-Antibody Affinity,
pubmed-meshheading:103569-Antigen-Antibody Reactions,
pubmed-meshheading:103569-Binding Sites, Antibody,
pubmed-meshheading:103569-Cyanogen Bromide,
pubmed-meshheading:103569-Galactosidases,
pubmed-meshheading:103569-Peptide Fragments,
pubmed-meshheading:103569-Protein Conformation,
pubmed-meshheading:103569-Trypsin,
pubmed-meshheading:103569-beta-Galactosidase
|
|
pubmed:year |
1978
|
|
pubmed:articleTitle |
Probes of beta-galactosidase structure with antibodies. Reaction of anti-peptide antibodies against native enzyme.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
