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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1999-8-2
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pubmed:databankReference |
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pubmed:abstractText |
We describe a novel chick WD-protein, cSWiP-1, expressed in somitic mesoderm and developing limb buds as well as in other embryonic structures where Hedgehog signalling has been shown to play a role. Using embryonic manipulations we show that in somites cSWiP-1 expression integrates two signals originating from structures adjacent to the segmental mesoderm: a positive signal from the notochord and a negative signal from intermediate and/or lateral mesoderm. In explant cultures of somitic mesoderm, Shh protein induces cSWiP-1, while a blocking antibody to Shh inhibits the induction of cSWiP-1 by the notochord. These results show that the positive signal from the notochord is mediated by Shh. We also show that in limb buds cSWiP-1 is upregulated by ectopic Shh. This occurs in about the same time period as upregulation of BMP2, placing cSWiP-1 among the earliest markers for the change of limb pattern caused by ectopic Shh. We also describe a human homologue of cSWiP-1 and a mouse gene, mSWiP-2, that is more distantly related to SWiP-1, suggesting that SWiP-1 belongs to a novel subfamily of WD-proteins.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BMP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Bmp2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 2,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hedgehog Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SHH protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0925-4773
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
79-94
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10354473-Amino Acid Sequence,
pubmed-meshheading:10354473-Animals,
pubmed-meshheading:10354473-Bone Morphogenetic Protein 2,
pubmed-meshheading:10354473-Bone Morphogenetic Proteins,
pubmed-meshheading:10354473-Chick Embryo,
pubmed-meshheading:10354473-Embryonic Induction,
pubmed-meshheading:10354473-Extremities,
pubmed-meshheading:10354473-Gene Expression Regulation, Developmental,
pubmed-meshheading:10354473-Hedgehog Proteins,
pubmed-meshheading:10354473-Humans,
pubmed-meshheading:10354473-Mice,
pubmed-meshheading:10354473-Molecular Sequence Data,
pubmed-meshheading:10354473-Notochord,
pubmed-meshheading:10354473-Proteins,
pubmed-meshheading:10354473-Sequence Homology, Amino Acid,
pubmed-meshheading:10354473-Signal Transduction,
pubmed-meshheading:10354473-Somites,
pubmed-meshheading:10354473-Species Specificity,
pubmed-meshheading:10354473-Trans-Activators,
pubmed-meshheading:10354473-Transforming Growth Factor beta
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pubmed:year |
1999
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pubmed:articleTitle |
SWiP-1: novel SOCS box containing WD-protein regulated by signalling centres and by Shh during development.
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pubmed:affiliation |
Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, 701 West 168th Street, New York, NY 10032, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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