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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-5-25
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pubmed:abstractText |
We examined the effect of ultraviolet (UV) irradiation on the expression of cyclooxygenases in cultured HaCaT keratinocytes and in human skin in vivo. UVB irradiation (10 and 50 mJ/cm2) and hydrogen peroxide (200 micromol/L) increased cyclooxygenase-2 mRNA expression in HaCaT keratinocytes. No clear expression of cyclooxygenase-1 mRNA was detected in either control or stimulated HaCaT cells. Genistein, a tyrosine kinase inhibitor, suppressed both the basal and stimulated expression of cyclooxygenase-2 in HaCaT cells. UVB-induced cyclooxygenase-2 mRNA expression was partly inhibited by the antioxidant N-acetylcysteine and by H-7, a non-specific inhibitor of protein kinase C. Solar-simulated irradiation (40 mJ/cm2) was found to induce in vivo both cyclooxygenase-2 mRNA and protein expression in human skin, whereas the expression of cyclooxygenase-1 mRNA remained at the basal level. Our results show that cyclooxygenase-2 expression is induced by UV irradiation and suggest that tyrosine kinases and reactive oxygen intermediates are involved in this induction of cyclooxygenase-2.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(5-Isoquinolinesulfonyl)-2-Methylp...,
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Genistein,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidants,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoprotein Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Vanadates
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0007-0963
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
140
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1017-22
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10354065-1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine,
pubmed-meshheading:10354065-Acetylcysteine,
pubmed-meshheading:10354065-Adult,
pubmed-meshheading:10354065-Antioxidants,
pubmed-meshheading:10354065-Blotting, Northern,
pubmed-meshheading:10354065-Cell Line,
pubmed-meshheading:10354065-Cyclooxygenase 1,
pubmed-meshheading:10354065-Cyclooxygenase 2,
pubmed-meshheading:10354065-Enzyme Inhibitors,
pubmed-meshheading:10354065-Epidermis,
pubmed-meshheading:10354065-Gene Expression,
pubmed-meshheading:10354065-Genistein,
pubmed-meshheading:10354065-Humans,
pubmed-meshheading:10354065-Hydrogen Peroxide,
pubmed-meshheading:10354065-Immunohistochemistry,
pubmed-meshheading:10354065-Isoenzymes,
pubmed-meshheading:10354065-Keratinocytes,
pubmed-meshheading:10354065-Male,
pubmed-meshheading:10354065-Membrane Proteins,
pubmed-meshheading:10354065-Oxidants,
pubmed-meshheading:10354065-Phosphoprotein Phosphatases,
pubmed-meshheading:10354065-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:10354065-Protein Kinase C,
pubmed-meshheading:10354065-Protein-Tyrosine Kinases,
pubmed-meshheading:10354065-RNA, Messenger,
pubmed-meshheading:10354065-Ultraviolet Rays,
pubmed-meshheading:10354065-Vanadates
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pubmed:year |
1999
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pubmed:articleTitle |
Ultraviolet irradiation induces cyclooxygenase-2 expression in keratinocytes.
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pubmed:affiliation |
Department of Clinical Chemistry, University of Turku, Turku, Finland. kirsi.isoherranen@huch.fi
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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