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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1999-7-26
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pubmed:databankReference |
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pubmed:abstractText |
The N-terminus of human topoisomerase I participates in the binding of this enzyme to helicases and other proteins. Using the N-terminal 250 amino acids of human topoisomerase I and a yeast two-hybrid/ in vitro binding screen, a novel arginine-serine-rich peptide was identified as a human topoisomerase I-binding protein. The corresponding full-length protein, named topors, contains a consensus RING zinc finger domain and nuclear localization signals in addition to the arginine-serine-rich region. The RING finger domain of topors is homologous to a similar domain in a family of viral proteins that are involved in the regulation of viral transcription. When expressed in HeLa cells as a green fluorescent protein fusion, topors localizes in the nucleus in a punctate pattern and co-immunoprecipitates with topoisomerase I. These data suggest that topors is involved in trans-cription, possibly recruiting topoisomerase I to RNA polymerase II transcriptional complexes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2538-44
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pubmed:dateRevised |
2008-11-20
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pubmed:meshHeading |
pubmed-meshheading:10352183-Amino Acid Sequence,
pubmed-meshheading:10352183-Carrier Proteins,
pubmed-meshheading:10352183-Cell Nucleus,
pubmed-meshheading:10352183-DNA, Complementary,
pubmed-meshheading:10352183-DNA Topoisomerases, Type I,
pubmed-meshheading:10352183-DNA-Binding Proteins,
pubmed-meshheading:10352183-HeLa Cells,
pubmed-meshheading:10352183-Humans,
pubmed-meshheading:10352183-Molecular Sequence Data,
pubmed-meshheading:10352183-Neoplasm Proteins,
pubmed-meshheading:10352183-Nuclear Proteins,
pubmed-meshheading:10352183-Precipitin Tests,
pubmed-meshheading:10352183-Saccharomyces cerevisiae,
pubmed-meshheading:10352183-Sequence Homology, Amino Acid,
pubmed-meshheading:10352183-Transcription Factors,
pubmed-meshheading:10352183-U937 Cells,
pubmed-meshheading:10352183-Ubiquitin-Protein Ligases,
pubmed-meshheading:10352183-Zinc Fingers
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pubmed:year |
1999
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pubmed:articleTitle |
Interaction between human topoisomerase I and a novel RING finger/arginine-serine protein.
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pubmed:affiliation |
Departments of Pharmacology and Medicine, Robert Wood Johnson Medical School, The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08901, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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