Linked Life Data

10352181

Source:http://linkedlifedata.com/resource/pubmed/id/10352181

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1999-7-26
pubmed:abstractText
Higher eukaryotes are proficient at remodeling palindromic DNA. As shown here, a fully palindromic 15.4 kb circular DNA can be introduced into rodent cells with the novel result that the molecule is reproducibly and site-specifically converted to a monomeric circle. The dimer-to-monomer conversion has not been described previously, and in particular is undetectable in Escherichia coli. Comparative DNA sequence analyses of the new junctions found within the monomer circles suggest that the resolution process involves formation of hairpin DNA structures followed by the introduction of single-strand nicks near their termini. By extension, hairpin nicking combined with non-homologous end-joining may be important as a general mechanism for the main-tenance of genomic stability in mammalian cells. It is suggested that the absence of a comparable strategy for coping with palindrome-induced structure in E.coli and other unicellular organisms underlies a fundamental difference in DNA sequence organiz-ation in the genomes of prokaryotes versus higher eukaryotes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2521-8
pubmed:dateRevised
2008-11-20
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Palindromy is eliminated through a structure-specific recombination process in rodent cells.
pubmed:affiliation
Program in Genetics and Genomic Biology, Hospital for Sick Children Research Institute and Department of Immunology, University of Toronto, Toronto, Canada. susanna.lewis@utoronto.ca
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't