Source:http://linkedlifedata.com/resource/pubmed/id/10350657
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1999-7-2
|
| pubmed:abstractText |
BCNT, named after Bucentaur, is a protein that contains a 324-amino-acid region derived from part of a long interspersed DNA sequence element (LINE) in Ruminantia. However, the unique portion is completely missing in human and mouse BCNTs. Since no significant information on their function has been obtained by homology search, we at first examined cellular localization and biochemical characteristics of bovine BCNT to get a hint on its function. Subcellular fractionation and immunohistochemical analyses using a normal bovine epithelial cell line and bovine brain revealed that a significant amount of bovine BCNT is localized in the nuclei, while the major portion is present in the cytosol. Furthermore, it was shown that bovine BCNT is a phosphoprotein and that both bovine and human BCNTs are phosphorylated by casein kinase II in vitro. These results show that BCNTs consist of a unique family, probably a substrate of casein kinase II, which may contribute further to the understanding of gene evolution.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0006-3002
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
24
|
| pubmed:volume |
1427
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
408-16
|
| pubmed:dateRevised |
2006-5-12
|
| pubmed:meshHeading |
pubmed-meshheading:10350657-Amino Acid Sequence,
pubmed-meshheading:10350657-Animals,
pubmed-meshheading:10350657-Brain,
pubmed-meshheading:10350657-Cattle,
pubmed-meshheading:10350657-Cell Fractionation,
pubmed-meshheading:10350657-Cell Line,
pubmed-meshheading:10350657-Cell Nucleus,
pubmed-meshheading:10350657-DNA,
pubmed-meshheading:10350657-Deer,
pubmed-meshheading:10350657-Immunohistochemistry,
pubmed-meshheading:10350657-Kidney,
pubmed-meshheading:10350657-Liver,
pubmed-meshheading:10350657-Molecular Sequence Data,
pubmed-meshheading:10350657-Phosphoproteins,
pubmed-meshheading:10350657-Precipitin Tests,
pubmed-meshheading:10350657-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:10350657-Ruminants,
pubmed-meshheading:10350657-Sequence Homology, Amino Acid
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Partial nuclear localization of a bovine phosphoprotein, BCNT, that includes a region derived from a LINE repetitive sequence in Ruminantia.
|
| pubmed:affiliation |
Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194, Japan. siwast@libra.ls.m-kagaku.co.jp
|
| pubmed:publicationType |
Journal Article
|