Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1999-6-28
pubmed:abstractText
We have determined that the genes encoding the secreted proteins EspA, EspD, and EspB of enterohemorrhagic Escherichia coli (EHEC) are organized in a single operon. The esp operon is controlled by a promoter located 94 bp upstream from the ATG start codon of the espA gene. The promoter is activated in the early logarithmic growth phase, upon bacterial contact with eukaryotic cells and in response to Ca2+, Mn2+, and HEPES. Transcription of the esp operon seems to be switched off in tightly attached bacteria. The activation process is regulated by osmolarity (induction at high osmolarities), modulated by temperature, and influenced by the degree of DNA supercoiling. Transcription is sigmaS dependent, and the H-NS protein contributes to its fine tuning. Identification of the factors involved in activation of the esp operon and the signals responsible for modulation may facilitate understanding of the underlying molecular events leading to sequential expression of virulence factors during natural infections caused by EHEC.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-10066458, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-1406272, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-1552854, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-1648559, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-1682258, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-1729202, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-1997427, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-2160266, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-2171779, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-2475391, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-2744487, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-2984680, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-3528323, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-6350186, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-7543880, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-7609755, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-7729884, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-7867068, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-7878036, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-8021202, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-8416901, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-8475100, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-8561457, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-8733230, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-8830276, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-8861207, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-8885260, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-8890194, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-8890245, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-8940434, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9080609, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9140961, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9141189, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9184008, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9367747, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9379902, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9390560, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9457432, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9495751, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9545230, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9632251, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9721271, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9746560, http://linkedlifedata.com/resource/pubmed/commentcorrection/10348852-9786192
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Superhelical, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins, http://linkedlifedata.com/resource/pubmed/chemical/EspA protein, E coli, http://linkedlifedata.com/resource/pubmed/chemical/H-NS protein, bacteria, http://linkedlifedata.com/resource/pubmed/chemical/HEPES, http://linkedlifedata.com/resource/pubmed/chemical/Manganese, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial, http://linkedlifedata.com/resource/pubmed/chemical/Sigma Factor, http://linkedlifedata.com/resource/pubmed/chemical/sigma factor KatF protein, Bacteria
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
181
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3409-18
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:10348852-Humans, pubmed-meshheading:10348852-Hydrogen-Ion Concentration, pubmed-meshheading:10348852-Calcium, pubmed-meshheading:10348852-Temperature, pubmed-meshheading:10348852-Manganese, pubmed-meshheading:10348852-Base Sequence, pubmed-meshheading:10348852-Genes, Bacterial, pubmed-meshheading:10348852-RNA, Bacterial, pubmed-meshheading:10348852-Bacterial Proteins, pubmed-meshheading:10348852-Amino Acid Sequence, pubmed-meshheading:10348852-Osmolar Concentration, pubmed-meshheading:10348852-HeLa Cells, pubmed-meshheading:10348852-Molecular Sequence Data, pubmed-meshheading:10348852-Transcription, Genetic, pubmed-meshheading:10348852-Operon, pubmed-meshheading:10348852-Bacterial Adhesion, pubmed-meshheading:10348852-Escherichia coli Proteins, pubmed-meshheading:10348852-Gene Expression Regulation, Bacterial, pubmed-meshheading:10348852-Promoter Regions, Genetic, pubmed-meshheading:10348852-DNA-Binding Proteins, pubmed-meshheading:10348852-DNA, Superhelical, pubmed-meshheading:10348852-HEPES
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