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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1999-7-1
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pubmed:abstractText |
Trypanosome protozoa, an early lineage of eukaryotic cells, have proteases homologous to mammalian lysosomal cathepsins, but the precursor proteins lack mannose 6-phosphate. Utilizing green fluorescent protein as a reporter, we demonstrate that the carbohydrate-free prodomain of a trypanosome cathepsin L is necessary and sufficient for directing green fluorescent protein to the lysosome/endosome compartment. A proper prodomain/catalytic domain processing site sequence is also required to free the mature protease for delivery to the lysosome/endosome compartment. A nine-amino acid prodomain loop motif, implicated in prodomain-receptor interactions in mammalian cells, is conserved in the protozoa. Site-directed mutagenesis now confirms the importance of this loop to protease trafficking and suggests that a protein motif targeting signal for lysosomal proteases arose early in eukaryotic cell evolution.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
16249-56
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10347181-Amino Acid Sequence,
pubmed-meshheading:10347181-Amino Acid Substitution,
pubmed-meshheading:10347181-Animals,
pubmed-meshheading:10347181-Biological Transport,
pubmed-meshheading:10347181-Cell Compartmentation,
pubmed-meshheading:10347181-Cysteine Endopeptidases,
pubmed-meshheading:10347181-Enzyme Precursors,
pubmed-meshheading:10347181-Green Fluorescent Proteins,
pubmed-meshheading:10347181-Leishmania mexicana,
pubmed-meshheading:10347181-Luminescent Proteins,
pubmed-meshheading:10347181-Microscopy, Confocal,
pubmed-meshheading:10347181-Molecular Sequence Data,
pubmed-meshheading:10347181-Mutagenesis, Site-Directed,
pubmed-meshheading:10347181-Protozoan Proteins,
pubmed-meshheading:10347181-Sequence Alignment,
pubmed-meshheading:10347181-Structure-Activity Relationship,
pubmed-meshheading:10347181-Trypanosoma cruzi
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pubmed:year |
1999
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pubmed:articleTitle |
Protease trafficking in two primitive eukaryotes is mediated by a prodomain protein motif.
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pubmed:affiliation |
Department of Pathology, University of California, San Francisco, California 94121, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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