Source:http://linkedlifedata.com/resource/pubmed/id/10346918
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
21
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| pubmed:dateCreated |
1999-6-23
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| pubmed:abstractText |
This study is the first detailing drug-induced changes in EBV DNA replication intermediates (RIs). Both EBV replication inhibition and damage induction were studied in latently infected human Raji cells treated with the enediyne DNA strand-scission agent C-1027. Analysis of RIs on two-dimensional agarose gels revealed a rapid loss in the EBV bubble arc. When elongation of nascent chains was blocked by aphidicolin, this loss was inhibited, suggesting that C-1027-induced disappearance of RIs was related to maturation of preformed replication molecules in the absence of initiation of new RIs. C-1027 damage to EBV DNA was limited at concentrations where loss of the bubble arc was nearly complete, and none was detected within the replicating origin (ori P)-containing fragment, indicating that replication inhibition occurred in trans. By contrast, the non-nuclear mitochondrial genome was insensitive to replication inhibition but highly sensitive to damage induced by C-1027. C-1027-induced trans inhibition of nuclear but not mitochondrial DNA replication is consistent with a cell cycle checkpoint response to a DNA-damaging agent. EBV replication and Raji cell growth were inhibited at equivalent C-1027 doses.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aminoglycosides,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Antibiotics, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/C 1027,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Mitochondrial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Enediynes,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0006-2960
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
25
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| pubmed:volume |
38
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
6962-70
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:10346918-Aminoglycosides,
pubmed-meshheading:10346918-Anti-Bacterial Agents,
pubmed-meshheading:10346918-Antibiotics, Antineoplastic,
pubmed-meshheading:10346918-Cell Nucleus,
pubmed-meshheading:10346918-DNA, Mitochondrial,
pubmed-meshheading:10346918-DNA, Viral,
pubmed-meshheading:10346918-DNA Damage,
pubmed-meshheading:10346918-DNA Replication,
pubmed-meshheading:10346918-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:10346918-Enediynes,
pubmed-meshheading:10346918-Growth Inhibitors,
pubmed-meshheading:10346918-Herpesvirus 4, Human,
pubmed-meshheading:10346918-Humans,
pubmed-meshheading:10346918-Mitochondria,
pubmed-meshheading:10346918-Transcription, Genetic,
pubmed-meshheading:10346918-Tumor Cells, Cultured,
pubmed-meshheading:10346918-Virus Latency,
pubmed-meshheading:10346918-Virus Replication
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| pubmed:year |
1999
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| pubmed:articleTitle |
C-1027-induced alterations in Epstein-Barr viral DNA replication in latently infected cultured human Raji cells: relationship to DNA damage.
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| pubmed:affiliation |
Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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