Linked Life Data

10344260

Source:http://linkedlifedata.com/resource/pubmed/id/10344260

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4-5
pubmed:dateCreated
1999-7-29
pubmed:abstractText
Molecular mechanisms underlying the cystic degeneration of ovarian follicles in the dairy cow have not been clarified yet. A useful approach for complementing endocrinological and clinical studies could be represented by the systematic analysis of the protein patterns in follicular and cystic fluid. With this aim, a two-dimensional polyacrylamide gel electrophoresis map of proteins contained in fluid from bovine ovarian follicles at different stages of development and from bovine ovarian cyst has been obtained. About 200 spots were detected after silver staining. Polypeptides from nine spots or series of spots have been identified by N-terminal sequencing, and further analysis of the map has been performed by gel comparison. Alpha-1-antitrypsin, albumin, serotransferrin and apolipoprotein A-I and A-IV were located on the map. Comparison between protein patterns revealed the differential expression of some spots among follicles of smaller diameter, follicles of larger diameter, and cysts. This could represent the first step toward the identification of proteins differentially expressed and associated with ovarian cyst development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0173-0835
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
866-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Two-dimensional polyacrylamide gel electrophoresis map of bovine ovarian fluid proteins.
pubmed:affiliation
Istituto di Fisiologia Veterinaria e Biochimica, Università degli Studi di Milano, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't