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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1999-6-29
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pubmed:abstractText |
Oligosaccharides released from porcine thyroglobulin were first derivatised with 2-aminoacridone (2-AMAC) and analysed by capillary electrophoresis to determine the complexity of this glycan pool. The same glycan mixture was then subjected to either a sialidase digest or a sialidase and fucosidase digest prior to derivatisation with 2-AMAC and analysis by normal phase high pressure liquid chromatography (HPLC). Comparison of the three chromatographic profiles with known standards allowed an initial identification of the glycan structures. The 2-AMAC derivatised glycans were then collected from HPLC for matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) analysis and the molecular weights of predicted structures were confirmed. This study demonstrates that a two enzyme array and subsequent MALDI-TOF analysis can be used successfully to assign the major glycans present in a complex mixture.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0951-4198
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
716-23
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10343413-Aminoacridines,
pubmed-meshheading:10343413-Animals,
pubmed-meshheading:10343413-Chromatography, High Pressure Liquid,
pubmed-meshheading:10343413-Electrophoresis, Capillary,
pubmed-meshheading:10343413-Fluorescent Dyes,
pubmed-meshheading:10343413-Hydrolysis,
pubmed-meshheading:10343413-Neuraminidase,
pubmed-meshheading:10343413-Oligosaccharides,
pubmed-meshheading:10343413-Polysaccharides,
pubmed-meshheading:10343413-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:10343413-Swine,
pubmed-meshheading:10343413-Thyroglobulin,
pubmed-meshheading:10343413-alpha-L-Fucosidase
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pubmed:year |
1999
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pubmed:articleTitle |
A chromatographic and mass spectrometric strategy for the analysis of oligosaccharides: determination of the glycan structures in porcine thyroglobulin.
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pubmed:affiliation |
SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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