Source:http://linkedlifedata.com/resource/pubmed/id/10342283
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1999-7-27
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pubmed:abstractText |
Localization of metallothionein (MT) -I & -II and MT-III and its significance in the brain aging in dogs were examined using immunohistological and molecular pathological techniques. MT-I & -II immunohistochemistry showed positive staining in the hypertrophic astrocytes throughout the aged dog brains; these MT-I & -II immunoreactive astrocytes were predominant in the cerebral cortex and around the blood vessels in the brain. These findings dominated in the brain regions with severe age-related morphological changes. In situ hybridization using MT-I mRNA riboprobes also demonstrated signals for MT-I mRNA in these hypertrophic astrocytes. Immunohistochemistry using a guinea pig antiserum against a synthetic polypeptide of canine MT-III demonstrated positive MT-III immunoreactivity predominantly in neurons in the Zn-rich regions such as hippocampus and parahippocampus. The findings were supported by in situ hybridization using MT-III mRNA riboprobes. Both MT-III immunoreactivity and signals for MT-III mRNA were demonstrated in neurons in the brain regardless of the intensity of the age-related changes. These results suggest, first, MT-I & -II may be induced in relation to the progress of the age-related morphological changes in the brain, playing an important role in the protection of the brain tissue from the toxic insults responsible for the brain aging, and second, MT-III may play a role in maintenance of Zn-related essential functions of the brain.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0916-7250
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
343-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10342283-Aging,
pubmed-meshheading:10342283-Animals,
pubmed-meshheading:10342283-Blotting, Western,
pubmed-meshheading:10342283-Brain Chemistry,
pubmed-meshheading:10342283-Dogs,
pubmed-meshheading:10342283-Female,
pubmed-meshheading:10342283-In Situ Hybridization,
pubmed-meshheading:10342283-Male,
pubmed-meshheading:10342283-Metallothionein,
pubmed-meshheading:10342283-Nerve Tissue Proteins,
pubmed-meshheading:10342283-RNA, Messenger
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pubmed:year |
1999
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pubmed:articleTitle |
Localization of metallothioneins-I & -II and -III in the brain of aged dog.
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pubmed:affiliation |
Department of Veterinary Pathology, Tottori University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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