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rdf:type |
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lifeskim:mentions |
umls-concept:C0007600,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0018270,
umls-concept:C0027740,
umls-concept:C0033195,
umls-concept:C0185117,
umls-concept:C0334227,
umls-concept:C0684249,
umls-concept:C1522642,
umls-concept:C1711207,
umls-concept:C2911684
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pubmed:issue |
6
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pubmed:dateCreated |
1999-7-7
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pubmed:abstractText |
Nerve growth factor-induced clone B (NGFI-B), Nur-related factor 1, and neuron-derived orphan receptor-1 have structural features of ligand-activated transcriptional regulators and constitute the NGFI-B subfamily within the nuclear receptor superfamily. The NGFI-B subfamily is highly expressed in neuroendocrine organs and regulates the pro-opiomelanocortin (POMC) gene. Small-cell lung cancer (SCLC) is considered to be a neuroendocrine tumor that produces large numbers of polypeptide hormones. In this study we measured the NGFI-B subfamily and POMC messenger RNA (mRNA) levels in various lung-cancer cell lines by means of the quantitative reverse transcription-polymerase chain reaction, and evaluated the correlations between expression of these genes and polypeptide hormone productions. We also examined the effect of antisense oligonucleotide to NGFI-B mRNA on the expression of POMC mRNA. The NGFI-B subfamily and POMC mRNAs were highly expressed in SCLC cell lines. In addition, there were strong correlations between the NGFI-B, POMC genes, and the adrenocorticotropin hormone (ACTH) level. Further, the antisense oligonucleotide significantly suppressed POMC gene expression. We conclude that the NGFI-B subfamily was a significant molecule in SCLC and that the NGFI-B was a positive transcriptional factor for ACTH production.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NR4A1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/NR4A2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/NR4A3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Receptor Subfamily 4...,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Receptor Subfamily 4...,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Pro-Opiomelanocortin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Steroid,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thyroid Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1044-1549
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1319-25
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10340952-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:10340952-Carcinoma, Small Cell,
pubmed-meshheading:10340952-DNA-Binding Proteins,
pubmed-meshheading:10340952-Dose-Response Relationship, Drug,
pubmed-meshheading:10340952-Gene Expression,
pubmed-meshheading:10340952-Humans,
pubmed-meshheading:10340952-Lung Neoplasms,
pubmed-meshheading:10340952-Multigene Family,
pubmed-meshheading:10340952-Nerve Growth Factors,
pubmed-meshheading:10340952-Nerve Tissue Proteins,
pubmed-meshheading:10340952-Nuclear Receptor Subfamily 4, Group A, Member 1,
pubmed-meshheading:10340952-Nuclear Receptor Subfamily 4, Group A, Member 2,
pubmed-meshheading:10340952-Oligonucleotides, Antisense,
pubmed-meshheading:10340952-Pro-Opiomelanocortin,
pubmed-meshheading:10340952-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:10340952-Receptors, Steroid,
pubmed-meshheading:10340952-Receptors, Thyroid Hormone,
pubmed-meshheading:10340952-Transcription Factors,
pubmed-meshheading:10340952-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Expression of nerve growth factor-induced clone B subfamily and pro-opiomelanocortin gene in lung cancer cell lines.
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pubmed:affiliation |
First Department of Internal Medicine, Kagawa Medical University, Kagawa, Japan.
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pubmed:publicationType |
Journal Article
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