Source:http://linkedlifedata.com/resource/pubmed/id/10337661
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1999-7-19
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| pubmed:abstractText |
In order to clarify the biologic characteristics of serous papillary adenocarcinoma of the human ovary, we tried to establish a continuous cell line using four primary tumor specimens derived from four patients with such tumors. We also evaluated c-myc, MYCN and c-erbB2 gene amplification in the cultured cells, and the xenografts, as well as in these four primary tissue specimens by a Southern blot analysis. One continuous cell line, named as FU-OV-1, was established in a serum-free culture system and this line propagated continuously for 96 serial passages over a 2-year-period in vitro. FU-OV-1 reproduced and still maintained the characteristics of the original tumor. C-myc gene amplification was found in the FU-OV-1 cells, and the xenografts, as well as in only the primary tumor of FU-OV-1 out of the four primary serous papillary adenocarcinomas. However, neither MYCN amplification nor c-erbB2 amplification was observed in any tumor specimens including FU-OV-1 cells. In conclusion, FU-OV-1 is thus considered to be a useful system for studying the biological behavior of serous papillary adenocarcinoma in the human ovary. The results of this study suggest that c-myc gene amplification might thus be associated with the progression of this tumor both in vitro and in vivo.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0344-0338
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
195
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
237-42
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10337661-Adult,
pubmed-meshheading:10337661-Aged,
pubmed-meshheading:10337661-Animals,
pubmed-meshheading:10337661-Chromosome Aberrations,
pubmed-meshheading:10337661-Chromosome Disorders,
pubmed-meshheading:10337661-Clone Cells,
pubmed-meshheading:10337661-Culture Media, Serum-Free,
pubmed-meshheading:10337661-Cystadenocarcinoma, Papillary,
pubmed-meshheading:10337661-Female,
pubmed-meshheading:10337661-Gene Amplification,
pubmed-meshheading:10337661-Genes, erbB-2,
pubmed-meshheading:10337661-Genes, myc,
pubmed-meshheading:10337661-Humans,
pubmed-meshheading:10337661-Karyotyping,
pubmed-meshheading:10337661-Mice,
pubmed-meshheading:10337661-Mice, Nude,
pubmed-meshheading:10337661-Middle Aged,
pubmed-meshheading:10337661-Neoplasm Transplantation,
pubmed-meshheading:10337661-Ovarian Neoplasms,
pubmed-meshheading:10337661-Tumor Cells, Cultured
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Establishment and characterization of a serous papillary adenocarcinoma cell line of the human ovary in a serum-free culture.
|
| pubmed:affiliation |
Department of Obstetrics and Gynecology, Fukuoka University School of Medicine, Japan.
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| pubmed:publicationType |
Journal Article
|