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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1999-7-29
pubmed:abstractText
The present study examines the cellular distribution of the ryanodine receptor/channel (RyR) and inositol 1,4,5-trisphosphate receptor (InsP3R) subtypes in parotid acini. Using fluorescently labelled 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-3-propionic acid glycyl-ryanodine (BODIPYtrade mark-ryanodine) and confocal microscopy, RyRs were localized primarily to the perinuclear region (basal pole) of the acinar cell. Ryanodine, Ruthenium Red, cAMP and cADP ribose (cADPR) competed with BODIPY-ryanodine, resulting in a reduction in the fluorescence signal. However, inositol 1,4, 5-trisphosphate [Ins(1,4,5)P3] did not alter the binding of BODIPY-ryanodine. Using receptor-subtype-specific antisera, InsP3Rs (types I, II and III) were located predominantly in the apical pole of the parotid cell. The presence of these three subtypes was confirmed using reverse transcriptase PCR with RNA-specific oligonucleotide probes. Binding studies using a parotid cell-membrane fraction identified and characterized RyRs and InsP3Rs in terms of binding affinity (Kd) and maximum binding capacity (Bmax) and confirmed that cADPR displaces ryanodine from its binding sites. Ruthenium Red and 8-Br-cADP-ribose blocked Ca2+ release in permeabilized acinar cells in response to cADPR and cAMP or forskolin, whereas Ins(1,4,5)P3-induced Ca2+ release was unaffected. The localization of the RyRs and InsP3Rs in discrete regions endow broad areas of the parotid cell with ligand-activated Ca2+ channels. The consequences of the dual activation of the RyRs and InsP3Rs by physiologically relevant stimuli such as noradrenaline (norepinephrine) are considered in relation to Ca2+ signalling in the parotid gland.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-1666327, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-2434495, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-2471423, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-2478025, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-5768873, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-6525621, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-7509390, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-7514529, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-7516436, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-7516645, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-7522674, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-7744807, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-7961809, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-8023884, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-8036248, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-8097730, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-8381210, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-8521483, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-8578608, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-8777135, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-8874493, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-8897836, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9083036, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9085309, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9130702, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9188460, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9188472, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9243320, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9354813, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9357775, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9382901, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-946932, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9480900, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9502195, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9575165, http://linkedlifedata.com/resource/pubmed/commentcorrection/10333498-9614187
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
340 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
519-27
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:10333498-Animals, pubmed-meshheading:10333498-Base Sequence, pubmed-meshheading:10333498-Calcium, pubmed-meshheading:10333498-Calcium Channels, pubmed-meshheading:10333498-DNA Primers, pubmed-meshheading:10333498-Fluorescent Antibody Technique, pubmed-meshheading:10333498-Inositol 1,4,5-Trisphosphate Receptors, pubmed-meshheading:10333498-Inositol Phosphates, pubmed-meshheading:10333498-Microscopy, Confocal, pubmed-meshheading:10333498-Parotid Gland, pubmed-meshheading:10333498-Radioligand Assay, pubmed-meshheading:10333498-Rats, pubmed-meshheading:10333498-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:10333498-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10333498-Ryanodine, pubmed-meshheading:10333498-Ryanodine Receptor Calcium Release Channel
pubmed:year
1999
pubmed:articleTitle
Ryanodine and inositol trisphosphate receptors are differentially distributed and expressed in rat parotid gland.
pubmed:affiliation
Department of Pharmacology and Toxicology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY 14214, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.
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